Review Article

Cannabis sativa L.: A Review on Traditional Uses, Botany, Phytochemistry, and Pharmacological Aspects


Medicinal and aromatic plants have been one of the most important sources of medicine since the dawn of human civilization. Indigenous communities have used products from this plant in different conditions throughout history. Cannabis sativa L. is one of the most widely employed herbaceous medicinal plants for textiles, and fibers, in medicine, as a source of food, animal food, animal bedding, and agriculture for seeds. This paper highlights the traditional applications, botany, phytochemistry, and pharmacological properties of C. sativa.  Extensive database retrieval, such as Google Scholar, Semantic Scholar, ResearchGate,, PubMed, SciFinder, ChemSpider, CNKI, PubFacts, etc., was performed by using the keywords “Hemp,” “Cannabis,” as well as the scientific name of this plant species (Cannabis sativa L). Besides, reviews of relevant textbooks, documents, and patents were also employed to collect sufficient information. This study revealed numerous pharmacological activities of C. sativa that could help with several medical diseases. Besides that, more than 565 bioactive constituents have been isolated and identified from diverse parts of C. sativa. This could help discover potential therapeutic effects and develop new medications to benefit human health.

Addlesperger E. Hemp. J Agric Food Inf 2015;16:196‑202.

Crini G, Lichtfouse E. Sustainable Agriculture Reviews 42: Hemp Production and Applications Vol. 42. Springer Nature. 2020.

Wills S. Cannabis use and abuse by man: an historical perspective. In: Cannabis. CRC Press 1998; pp 16‑46.

Conrad C. Hemp for health: The medicinal and nutritional uses of Cannabis sativa. Inner Traditions/Bear & Co. 1997.

Balant M, Gras A, Ruz M, Vallès J, Vitales D, et al. Traditional uses of Cannabis: An analysis of the CANNUSE database. J Ethnopharmacol 2021;279:114362.

Robinson R. The great book of hemp: the complete guide to the environmental, commercial, and medicinal uses of the world’s most extraordinary plant. Inner Traditions/Bear & Co. 1996.

Ranalli P, Venturi G. Hemp as a raw material for industrial applications. Euphytica 2004;140:1‑6.

Bouloc P. Hemp: industrial production and uses. CABI. 2013.

Talebi M, Zarshenas MM, Yazdani E, Moein M. Preparation and evaluation of possible antioxidant activities of rose traditional tablet “(Qurs-e-Vard)” a selected traditional persian medicine (TPM) formulation via various procedures. Curr Drug Discov Technol 2021;18:90‑97.

Talebi M, Kakouri E, Talebi M, Tarantilis PA, Farkhondeh T, et al. Nutraceuticals-based therapeutic approach: Recent advances to combat pathogenesis of Alzheimer’s disease. Expert Rev Neurother 2021;21:625‑642.

Porter AC, Felder CC. The endocannabinoid nervous system: unique opportunities for therapeutic intervention. Pharmacol Ther 2001;90:45‑60.

ElSohly MA. Chemical constituents of cannabis. Haworth Press. New York 2002.

Andre CM, Hausman JF, Guerriero G. Cannabis sativa: the plant of the thousand and one molecules. Front Plant Sci 2016;7:19.

Adejumo AC, Alliu S, Ajayi TO, Adejumo KL, Adegbala OM, et al. Cannabis use is associated with reduced prevalence of non-alcoholic fatty liver disease: A cross-sectional study. PloS One 2017;12:e0176416.

Argueta DA, Aich A, Muqolli F, Cherukury H, Sagi V, et al. Considerations for cannabis use to treat pain in sickle cell disease. J Clin Med 2020;9:3902.

Grotenhermen F, Müller-Vahl K. The therapeutic potential of cannabis and cannabinoids. Dtsch Ärztebl Int 2012;109:495.

Fleming MP, Clarke RC. Physical evidence for the antiquity of Cannabis sativa L. J Int Hemp Assoc 1998;5:80‑95.

Aldrich M. History of therapeutic cannabis. Cannabis Med Pract. Jefferson NC Mc Farland. 1997; pp 35‑55.

Small E. Cannabis: a complete guide. CRC Press. 2016.

Chandra S, Lata H, ElSohly MA. Cannabis sativa L.-botany and biotechnology. Springer. 2017.

Kwai IUN. Reclassifies cannabis as a less dangerous drug. N Y Times 2020;2:1‑3.

The Plant List. The Plant List. Version 1.1. Published on the Internet; (accessed 1st January). 2013.

ITIS. Integrated Taxonomic Information System (ITIS) on-line database,, CC0 [Internet]. 2022. Disponible sur:

Chaachouay N, Douira A, Hassikou R, Brhadda N, Dahmani J, et al. Floristic and ethnomedicinal study of aromatic and medicinal plants in the Rif (northern Morocco)" [Internet] [phd thesis]. 2020 [cited 18 Oct 2021]. Available from:

Bellakhdar J. Contribution to the study of the traditional pharmacopoeia in Morocco: the current situation, the products, the sources of knowledge (ethnopharmacological field survey carried out from 1969 to 1992) [PhD Thesis]. Paul Verlaine-Metz University; 1997.

Akbar S. Handbook of 200 medicinal plants: A comprehensive review of their traditional medical uses and scientific justifications. 2020.

CABI. Compendium Coordinator Invasive Species Compendium, CAB International, Wallingford; Oxon OX10 8DE, UK; Fax: +44 (0)1491 833508, Email: [Internet]. 2022. Disponible sur:

Ainsworth C. Boys and girls come out to play: the molecular biology of dioecious plants. Ann Bot 2000;86:211‑221.

Small E, Pocock T, Cavers PB. The biology of Canadian weeds. 119. Cannabis sativa L. Can J Plant Sci 2003;83:217‑237.

Chaohua C, Gonggu Z, Lining Z, Chunsheng G, Qing T, et al. A rapid shoot regeneration protocol from the cotyledons of hemp (Cannabis sativa L.). Ind Crops Prod 2016;83:61‑65.

Kudo Y, Kobayashi M, Momohara A, Noshiro S, Nakamura T, et al. Radiocarbon dating of the fossil hemp fruits in the earliest Jomon period from the Okinoshima Site, Chiba, Japan. Jpn J Hist Bot 2009;17:27‑32.

Brown DT. Cannabis: the genus cannabis. CRC Press. 1998.

Hallam C. The international drug control regime and access to controlled medicines. Policy 2014.

Lande A. The single convention on narcotic drugs, 1961. Int Organ 1962;16:776‑797.

Pertwee RG. Handbook of cannabis. Oxford University Press. USA 2014.

Schleimer JP, Rivera-Aguirre AE, Castillo-Carniglia A, Laqueur HS, Rudolph KE, et al. Investigating how perceived risk and availability of marijuana relate to marijuana use among adolescents in Argentina, Chile, and Uruguay over time. Drug Alcohol Depend 2019;201:115‑126.

Lipnik-Štangelj M, Razinger B. A regulatory take on cannabis and cannabinoids for medicinal use in the European Union. Arch Ind Hyg Toxicol 2020;71:12‑18.

Youngers CA. The drug policy reform agenda in the Americas. IDC Brief Pap 2013.

Ransing R, de la Rosa PA, Pereira-Sanchez V, Handuleh JI, Jerotic S, et al. Current state of cannabis use, policies, and research across sixteen countries: cross-country comparisons and international perspectives. Trends Psychiatry Psychother 2022;44: e20210263.

Cox C. The Canadian cannabis act legalizes and regulates recreational cannabis use in 2018. Health Policy 2018;122:205‑209.

Chapman SA, Spetz J, Lin J, Chan K, Schmidt LA. Capturing heterogeneity in medical marijuana policies: A taxonomy of regulatory regimes across the United States. Subst Use Misuse 2016;51:1174‑84.

Aguilar S, Gutiérrez V, Sánchez L, Nougier M. Medicinal cannabis policies and practices around the world. Int Drug Policy Consort Brief Pap 2018.

Ammerman S, Ryan S, Adelman WP, Levy S, Ammerman SD, et al. The impact of marijuana policies on youth: clinical, research, and legal update. Pediatrics 2015;135:e769‑e785.

Trivers KF, Phillips E, Gentzke AS, Tynan MA, Neff LJ. Prevalence of cannabis use in electronic cigarettes among US youth. JAMA Pediatr 2018;172:1097‑1099.

Schlag AK. An evaluation of regulatory regimes of medical cannabis: what lessons can be learned for the UK? Med Cannabis Cannabinoids 2020;3:76‑83.

Mamo JJ. Cannabis in Malta-big business or laxing restrictions? Eur J Public Health 2019;29:390‑391.

Lozano P, Barrientos-Gutierrez I, Arillo-Santillan E, Morello P, Mejia R, et al. A longitudinal study of electronic cigarette use and onset of conventional cigarette smoking and marijuana use among Mexican adolescents. Drug Alcohol Depend 2017;180:427‑430.

Seddon T, Floodgate W. Regulating Cannabis. Springer. 2020.

Wilkins C. A “not-for-profit” regulatory model for legal recreational cannabis: Insights from the regulation of gaming machine gambling in New Zealand. Int J Drug Policy 2018;53:115‑122.

Bridgeman MB, Abazia DT. Medicinal cannabis: history, pharmacology, and implications for the acute care setting. Pharm Ther 2017;42:180-188.

Blickman T. Cannabis policy reform in Europe. Bottom up rather than top down. Ser Legis Reform Drug Policies 2014;28.

Degenhardt L, Chiu WT, Sampson N, Kessler RC, Anthony JC, et al. Toward a global view of alcohol, tobacco, cannabis, and cocaine use: findings from the WHO World Mental Health Surveys. PLoS Med 2008;5:e141.

Leung J, Chiu CYV, Stjepanović D, Hall W. Has the legalisation of medical and recreational cannabis use in the USA affected the prevalence of cannabis use and cannabis use disorders? Curr Addict Rep 2018;5:403‑417.

Schultes RE, Klein WM, Plowman T, Lockwood TE. Cannabis: an example of taxonomic neglect. In: Cannabis and culture. De Gruyter Mouton 2011; pp 21‑38.

Bonini SA, Premoli M, Tambaro S, Kumar A, Maccarinelli G, et al. Cannabis sativa: A comprehensive ethnopharmacological review of a medicinal plant with a long history. J Ethnopharmacol 2018;227:300‑315.

Maule WJ. Medical uses of marijuana (Cannabis sativa): fact or fallacy? Br J Biomed Sci 2015;72:85‑91.

Pourbagher-Shahri AM, Farkhondeh T, Talebi M, Kopustinskiene DM, Samarghandian S, et al. An overview of NO signaling pathways in aging. Molecules 2021;26:4533.

Manzanares J, Julian MD, Carrascosa A. Role of the cannabinoid system in pain control and therapeutic implications for the management of acute and chronic pain episodes. Curr Neuropharmacol 2006;4:239‑257.

Schlosburg JE, Kinsey SG, Lichtman AH. Targeting fatty acid amide hydrolase (FAAH) to treat pain and inflammation. AAPS J 2009;11:39‑44.

Comelli F, Giagnoni G, Bettoni I, Colleoni M, Costa B. Antihyperalgesic effect of a Cannabis sativa extract in a rat model of neuropathic pain: mechanisms involved. Phytother Res 2008;22:1017‑1024.

Garg G, Adams JD. Treatment of neuropathic pain with plant medicines. Chin J Integr Med 2012;18:565‑570.

Berman JS, Symonds C, Birch R. Efficacy of two cannabis based medicinal extracts for relief of central neuropathic pain from brachial plexus avulsion: results of a randomised controlled trial. Pain 2004;112:299‑306.

Abrams DI, Guzman M. Cannabis in cancer care. Clin Pharmacol Ther 2015;97:575‑586.

Cassano T, Villani R, Pace L, Carbone A, Bukke VN, et al. From Cannabis sativa to cannabidiol: Promising therapeutic candidate for the treatment of neurodegenerative diseases. Front Pharmacol 2020;11:124.

Fleury-Teixeira P, Caixeta FV, Ramires da Silva LC, Brasil-Neto JP, Malcher-Lopes R. Effects of CBD-enriched Cannabis sativa extract on autism spectrum disorder symptoms: an observational study of 18 participants undergoing compassionate use. Front Neurol 2019;10:1145.

Grigoriev OV. Application of hempseed (Cannabis sativa L.) oil in the treatment of ear, nose and throat (ENT) disorders. J Ind Hemp 2002;7:5‑15.

Schicho R, Storr M. Cannabis finds its way into treatment of Crohn’s disease. Pharmacology 2014;93:1‑3.

Antonsdottir IM, Makino KM, Porsteinsson AP. Dazed and confused: medical cannabis in Alzheimer disease. Vol. 24, The American Journal of Geriatric Psychiatry. Elsevier 2016; pp 1004‑1006.

Gobira PH, Vilela LR, Goncalves BD, Santos RP, de Oliveira AC, et al. Cannabidiol, a Cannabis sativa constituent, inhibits cocaine-induced seizures in mice: possible role of the mTOR pathway and reduction in glutamate release. Neurotoxicology 2015;50:116‑121.

Martin BR, Wiley JL. Mechanism of action of cannabinoids: how it may lead to treatment of cachexia, emesis, and pain. J Support Oncol 2004;2:305‑314.

Kuhathasan N, Minuzzi L, MacKillop J, Frey BN. The Use of Cannabinoids for Insomnia in Daily Life: Naturalistic Study. J Med Internet Res 2021;23:e25730.

Chaachouay N, Zidane L. Neurological Phytotherapy by Indigenous People of Rif, Morocco. In: Therapy Approaches in Neurological Disorders. IntechOpen. 2021.

Chaachouay N, Benkhnigue O, Zidane L. Ethnobotanical study aimed at investigating the use of medicinal plants to treat nervous system diseases in the Rif of Morocco. J Chiropr Med 2020;19:70‑81.

Fragoso YD, Carra A, Macias MA. Cannabis and multiple sclerosis. Expert Rev Neurother 2020;20:849‑854.

Vaney C, Heinzel-Gutenbrunner M, Jobin P, Tschopp F, Gattlen B, et al. Efficacy, safety and tolerability of an orally administered cannabis extract in the treatment of spasticity in patients with multiple sclerosis: a randomized, double-blind, placebo-controlled, crossover study. Mult Scler J 2004;10:417‑424.

Zaka M, Sehgal SA, Shafique S, Abbasi BH. Comparative in silico analyses of Cannabis sativa, Prunella vulgaris and Withania somnifera compounds elucidating the medicinal properties against rheumatoid arthritis. J Mol Graph Model 2017;74:296‑304.

Lozano I. The therapeutic use of Cannabis sativa (L.) in Arabic medicine. J Cannabis Ther 2001;1:63‑70.

Russo EB, Grotenhermen F. The Therapeutic Use of Cannabis sativa (L.) in Arabic Medicine. In: The Handbook of Cannabis Therapeutics. Routledge 2014; pp 23‑30.

Peschel W. Quality control of traditional cannabis tinctures: pattern, markers, and stability. Sci Pharm 2016;84:567‑584.

Politeo O, Jukic M, Milos M. Comparison of chemical composition and antioxidant activity of glycosidically bound and free volatiles from clove (Eugenia caryophyllata Thunb.). J Food Biochem 2010;34:129‑141.

Shah NC. Indigenous uses and ethnobotany of Cannabis sativa L.(Hemp) in Uttaranchal (India). J Ind Hemp 2004;9:69‑77.

Jones K. Nutritional and medicinal guide to hemp seed. Rainforest Botanical Laboratory. 1995.

Salentijn EM, Zhang Q, Amaducci S, Yang M, Trindade LM. New developments in fiber hemp (Cannabis sativa L.) breeding. Ind Crops Prod 2015;68:32‑41.

Taura F, Sirikantaramas S, Shoyama Y, Yoshikai K, Shoyama Y, et al. Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa. FEBS Lett 2007;581:2929‑2934.

Väisänen T, Batello P, Lappalainen R, Tomppo L. Modification of hemp fibers (Cannabis sativa L.) for composite applications. Ind Crops Prod 2018;111:422‑429.

Ahmed SA, Ross SA, Slade D, Radwan MM, Khan IA, et al. Minor oxygenated cannabinoids from high potency Cannabis sativa L. Phytochemistry 2015;117:194‑199.

Rock EM, Parker LA. Constituents of Cannabis sativa. In: Cannabinoids and Neuropsychiatric Disorders. Springer 2021; pp 1‑13.

Brenneisen R. Chemistry and analysis of phytocannabinoids and other Cannabis constituents. In: Marijuana and the Cannabinoids. Springer 2007. pp 17‑49.

Calvi L, Pentimalli D, Panseri S, Giupponi L, Gelmini F, et al. Comprehensive quality evaluation of medical Cannabis sativa L. inflorescence and macerated oils based on HS-SPME coupled to GC–MS and LC-HRMS (q-exactive orbitrap®) approach. J Pharm Biomed Anal 2018;150:208‑219.

Radwan MM, Chandra S, Gul S, ElSohly MA. Cannabinoids, phenolics, terpenes and alkaloids of cannabis. Molecules 2021;26:2774.

Gertsch J, Pertwee RG, Di Marzo V. Phytocannabinoids beyond the Cannabis plant–do they exist? Br J Pharmacol 2010;160:523‑529.

Ibrahim EA, Wang M, Radwan MM, Wanas AS, Majumdar CG, et al. Analysis of terpenes in Cannabis sativa L. using GC/MS: method development, validation, and application. Planta Med 2019;85:431‑438.

Mudge EM, Brown PN, Murch SJ. The Terroir of Cannabis: terpene metabolomics as a tool to understand Cannabis sativa selections. Planta Med 2019;85:781‑796.

Radwan MM, ElSohly MA, Slade D, Ahmed SA, Wilson L, et al. Non-cannabinoid constituents from a high potency Cannabis sativa variety. Phytochemistry 2008;69:2627‑2633.

Haller VL, Cichewicz DL, Welch SP. Non-cannabinoid CB1, non-cannabinoid CB2 antinociceptive effects of several novel compounds in the PPQ stretch test in mice. Eur J Pharmacol 2006;546:60‑68.

Lowe H, Steele B, Bryant J, Toyang N, Ngwa W. Non-cannabinoid metabolites of Cannabis sativa L. With therapeutic potential. Plants 2021;10:400.

Gieringer D, St. Laurent J, Goodrich S. Cannabis vaporizer combines efficient delivery of THC with effective suppression of pyrolytic compounds. J Cannabis Ther 2004;4:7‑27.

Morris EM, Kitts-Morgan SE, Spangler DM, Gebert J, Vanzant ES, et al. Feeding Cannabidiol (CBD)-Containing Treats Did Not Affect Canine Daily Voluntary Activity. Front Vet Sci 2021;8.

Watt G, Karl T. In vivo evidence for therapeutic properties of cannabidiol (CBD) for Alzheimer’s disease. Front Pharmacol 2017;8:20.

Ligresti A, Moriello AS, Starowicz K, Matias I, Pisanti S, et al. Antitumor activity of plant cannabinoids with emphasis on the effect of cannabidiol on human breast carcinoma. J Pharmacol Exp Ther 2006;318:1375‑1387.

Crane NA, Schuster RM, Fusar-Poli P, Gonzalez R. Effects of cannabis on neurocognitive functioning: recent advances, neurodevelopmental influences, and sex differences. Neuropsychol Rev 2013;23:117‑137.

Pellati F, Borgonetti V, Brighenti V, Biagi M, Benvenuti S, et al. Cannabis sativa L. and nonpsychoactive cannabinoids: their chemistry and role against oxidative stress, inflammation, and cancer. BioMed Res Int 2018;2018.

Fukumoto S, Sawasaki E, Okuyama S, Miyake Y, Yokogoshi H. Flavor components of monoterpenes in citrus essential oils enhance the release of monoamines from rat brain slices. Nutr Neurosci 2006;9:73‑80.

Breitmaier E. Terpenes: Flavors, Fragrances, Pharmaca, Pheromones. John Wiley & Sons 2006; p 242.

Cox-Georgian D, Ramadoss N, Dona C, Basu C. Therapeutic and Medicinal Uses of Terpenes. In: Joshee N, Dhekney SA, Parajuli P, éditeurs. Medicinal Plants: From Farm to Pharmacy [Internet]. Cham: Springer International Publishing. 2019 [cité 21 juin 2022]. pp 333‑359. Disponible sur:

Perveen S, Al-Taweel A. Terpenes and Terpenoids. BoD – Books on Demand 2018; p 154.

Pollastro F, Minassi A, Fresu LG. Cannabis Phenolics and their Bioactivities. Curr Med Chem 2018;25:1160‑1185.

Soto-Hernández M, Tenango MP, García-Mateos R. Phenolic Compounds: Biological Activity. BoD – Books on Demand 2017; p 240.

Harborne JB. Phenolic Compounds. In: Harborne JB, éditeur. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis [Internet]. Dordrecht: Springer Netherlands 1973 [cité 21 juin 2022]. p. 33‑88. Disponible sur:

Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 2001;49:5165‑170.

ElSohly MA. Chemical constituents of cannabis. Haworth Press. New York 2002.

Turner CE, Elsohly MA, Boeren EG. Constituents of Cannabis sativa L. XVII. A review of the natural constituents. J Nat Prod 1980;43:169‑234.

ElSohly M, Gul W. Constituents of Cannabis sativa. Handb Cannabis 2014;3:1093.

ElSohly MA, Radwan MM, Gul W, Chandra S, Galal A. Phytochemistry of Cannabis sativa L. Prog Chem Org Nat Prod 2017;103:1‑36.

Turner CE, Hadley KW, Fetterman PS, Doorenbos NJ, Quimby MW, et al. Constituents of Cannabis sativa L. IV: Stability of cannabinoids in stored plant material. J Pharm Sci 1973;62:1601‑1605.

Van de Donk T, Niesters M, Kowal MA, Olofsen E, Dahan A, et al. An experimental randomized study on the analgesic effects of pharmaceutical-grade cannabis in chronic pain patients with fibromyalgia. Pain 2019;160:860-869.

Kraft B, Frickey NA, Kaufmann RM, Reif M, Frey R, et al. Lack of analgesia by oral standardized cannabis extract on acute inflammatory pain and hyperalgesia in volunteers. J Am Soc Anesthesiol 2008;109:101‑110.

Kosersky DS, Dewey WL, Harris LS. Antipyretic, analgesic and anti-inflammatory effects of Δ9-tetrahydrocannabinol in the rat. Eur J Pharmacol 1973;24:1‑7.

Formukong EA, Evans AT, Evans FJ. Analgesic and antiinflammatory activity of constituents of Cannabis sativa L. Inflammation 1988;12:361‑371.

Harris HM, Rousseau MA, Wanas AS, Radwan MM, Caldwell S, et al. Role of cannabinoids and terpenes in cannabis-mediated analgesia in rats. Cannabis Cannabinoid Res 2019;4:177‑182.

Sofia RD, Vassar HB, Knobloch LC. Comparative analgesic activity of various naturally occurring cannabinoids in mice and rats. Psychopharmacologia 1975;40:285‑295.

Buxbaum DM. Analgesic activity of Δ 9-tetrahydrocannabinol in the rat and mouse. Psychopharmacologia 1972;25:275‑280.

Guimarães FS, De Aguiar JC, Mechoulam R, Breuer A. Anxiolytic effect of cannabidiol derivatives in the elevated plus-maze. Gen Pharmacol 1994;25:161‑164.

Frassinetti S, Gabriele M, Moccia E, Longo V, Di Gioia D. Antimicrobial and antibiofilm activity of Cannabis sativa L. seeds extract against Staphylococcus aureus and growth effects on probiotic Lactobacillus spp. Lwt 2020;124:109149.

Friedman LK, Wongvravit JP. Anticonvulsant and neuroprotective effects of cannabidiol during the juvenile period. J Neuropathol Exp Neurol 2018;77:904‑919.

Hill AJ, Mercier MS, Hill TDM, Glyn SE, Jones NA, et al. Cannabidivarin is anticonvulsant in mouse and rat. Br J Pharmacol 2012;167:1629‑1642.

Karler R, Cely W, Turkanis SA. Anticonvulsant properties of Δ9-tetrahydrocannabinol and other cannabinoids. Life Sci 1974;15:931‑947.

Mc Caughran JA, Corcoran ME, Wada JA. Anticonvulsant activity of Δ8- and Δ9-tetrahydrocannabinol in rats. Pharmacol Biochem Behav 1974;2:227‑233.

Leite R, Carlini EA, Lander N, Mechoulam R. Anticonvulsant effects of the (–) and (+) isomers of cannabidiol and their dimethylheptyl homologs. Pharmacology 1982;24:141‑146.

Zanelati T, Biojone C, Moreira F, Guimarães F, Joca S. Antidepressant-like effects of cannabidiol in mice: possible involvement of 5-HT1A receptors: Cannabidiol induces antidepressant-like effect. Br J Pharmacol Janv 2010;159:122‑128.

Ahn Y, Han SH, Kim MG, Hong KB, Kim WJ, et al. Anti-depressant effects of ethanol extract from Cannabis sativa (hemp) seed in chlorpromazine-induced Drosophila melanogaster depression model. Pharm Biol 2021;59:998‑1007.

ElBatsh MM, Moklas MAA, Marsden CA, Kendall DA. Antidepressant-like effects of Δ9-tetrahydrocannabinol and rimonabant in the olfactory bulbectomised rat model of depression. Pharmacol Biochem Behav 2012;102:357‑365.

Orr Le, Mckernan Jf. Antiemetic effect of Δ9-tetrahydrocannabinol in chemotherapy-associated nausea and emesis as compared to placebo and compazine. J Clin Pharmacol 1981;21:76S-80S.

Khan IH, Javaid A. Antifungal activity of leaf extract of Cannabis sativa against Aspergillus flavipes. Pak J Weed Sci Res 2020;26:447-453.

Wanas AS, Radwan MM, Mehmedic Z, Jacob M, Khan IA, et al. Antifungal activity of the volatiles of high potency Cannabis sativa L. against Cryptococcus neoformans. Rec Nat Prod 2016;10:214-220.

Girgih AT, He R, Malomo S, Offengenden M, Wu J, et al. Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides. J Funct Foods 2014;6:384‑394.

Kosersky D. Antihypertensive effects of delta-9-tetrahydrocannabinol. Arch Int Pharmacodyn Ther 1978;233:76‑81.

Gallily R, Yekhtin Z, Hanuš LO. The anti-inflammatory properties of terpenoids from cannabis. Cannabis Cannabinoid Res 2018;3:282‑290.

Costa B, Colleoni M, Conti S, Parolaro D, Franke C, et al. Oral anti-inflammatory activity of cannabidiol, a non-psychoactive constituent of cannabis, in acute carrageenan-induced inflammation in the rat paw. Naunyn Schmiedebergs Arch Pharmacol 2004;369:294‑299.

Rajan TS, Giacoppo S, Iori R, De Nicola GR, Grassi G, et al. Anti-inflammatory and antioxidant effects of a combination of cannabidiol and moringin in LPS-stimulated macrophages. Fitoterapia 2016;112:104‑115.

Ali EMM, Almagboul AZI, Khogali SME, Gergeir UMA. Antimicrobial activity of Cannabis sativa. Chin Med 2012;3:61-64.

Palmieri S, Maggio F, Pellegrini M, Ricci A, Serio A, et al. Effect of the distillation time on the chemical composition, antioxidant potential and antimicrobial activity of essential oils from different Cannabis sativa L. cultivars. Molecules 2021;26:4770.

Tahsin KN, Watson D, Rizkalla A, Heinrichs D, Charpentier P. Antimicrobial studies of cannabidiol as biomaterials against superbug MRSA. CMBES Proc 2021;44.

Sarmadyan H, Solhi H, Najarian-Araghi N, Ghaznavi-Rad E. Determination of the antimicrobial effects of hydro-alcoholic extract‎ of Cannabis sativa on multiple drug resistant bacteria isolated from‎ nosocomial infections. Iran J Toxicol 2014;7:967‑972.

Muscarà C, Smeriglio A, Trombetta D, Mandalari G, La Camera E, et al. Antioxidant and antimicrobial activity of two standardized extracts from a new Chinese accession of non-psychotropic Cannabis sativa L. Phytother Res 2021;35:1099‑1112.

Martinenghi LD, Jønsson R, Lund T, Jenssen H. Isolation, purification, and antimicrobial characterization of cannabidiolic acid and cannabidiol from Cannabis sativa L. Biomolecules 2020;10:900.

Langezaal CR, Chandra A, Scheffer JJC. Antimicrobial screening of essential oils and extracts of some humulus lupulus L. Cultivars. Pharm World Sci 1992;14:353-356.

Teh SS, Bekhit AEDA, Carne A, Birch J. Antioxidant and ACE-inhibitory activities of hemp (Cannabis sativa L.) protein hydrolysates produced by the proteases AFP, HT, Pro-G, actinidin and zingibain. Food Chem 2016;203:199‑206.

Di Giacomo V, Chiavaroli A, Recinella L, Orlando G, Cataldi A, et al. Antioxidant and neuroprotective effects induced by cannabidiol and cannabigerol in rat CTX-TNA2 astrocytes and isolated cortexes. Int J Mol Sci 2020;21:3575.

Casares L, García V, Garrido-Rodríguez M, Millán E, Collado JA, et al. Cannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1. Redox Biol 2020;28:101321.

Yan X, Tang J, dos Santos Passos C, Nurisso A, Simoes-Pires CA, et al. Characterization of lignanamides from hemp (Cannabis sativa L.) seed and their antioxidant and acetylcholinesterase inhibitory activities. J Agric Food Chem 2015;63:10611‑106119.

Kornpointner C, Martinez AS, Marinovic S, Haselmair-Gosch C, Jamnik P, et al. Chemical composition and antioxidant potential of Cannabis sativa L. roots. Ind Crops Prod 2021;165:113422.

Moccia S, Siano F, Russo GL, Volpe MG, La Cara F, et al. Antiproliferative and antioxidant effect of polar hemp extracts (Cannabis sativa L., Fedora cv.) in human colorectal cell lines. Int J Food Sci Nutr 2020;71:410‑423.

Omer S, Boothe D, Mansour M, Dhanasekaran M, Pondugula S. Abstract PO-047: Anti-proliferative effect of cannabidiol (CBD) against B and T-cell lymphoma. AACR 2020.

Sainz-Cort A, Müller-Sánchez C, Espel E. Anti-proliferative and cytotoxic effect of cannabidiol on human cancer cell lines in presence of serum. BMC Res Notes 2020;13:1‑6.

Gercek OZ, Oflaz B, Oguz N, Demirci K, Gunduz O, et al. Role of nitric oxide in the antipruritic effect of win 55,212-2, a cannabinoid agonist. Basic Clin Neurosci 2020;11:473-480.

Bilir KA, Anli G, Ozkan E, Gunduz O, Ulugol A. Involvement of spinal cannabinoid receptors in the antipruritic effects of WIN 55,212-2, a cannabinoid receptor agonist. Clin Exp Dermatol 2018;43:553‑558.

Massi P, Vaccani A, Ceruti S, Colombo A, Abbracchio MP, et al. Antitumor effects of cannabidiol, a nonpsychoactive cannabinoid, on human glioma cell lines. J Pharmacol Exp Ther 2004;308:838‑845.

Todorova J, Lazarov LI, Petrova M, Tzintzarov A, Ugrinova I. The antitumor activity of cannabidiol on lung cancer cell lines A549 and H1299: the role of apoptosis. Biotechnol Biotechnol Equip 2021;35:873‑879.

Grijo DR, Bidoia DL, Nakamura CV, Osorio IV, Cardozo-Filho L. Analysis of the antitumor activity of bioactive compounds of Cannabis flowers extracted by green solvents. J Supercrit Fluids 2019;149:20‑25.

Schier AR de M, Ribeiro NP de O, Silva AC de O, Hallak JEC, Crippa JAS, et al. Cannabidiol, a Cannabis sativa constituent, as an anxiolytic drug. Braz J Psychiatry 2012;34:104‑110.

R de Mello Schier A, P de Oliveira Ribeiro N, S Coutinho D, Machado S, Arias-Carrión O, et al. Antidepressant-like and anxiolytic-like effects of cannabidiol: A chemical compound of Cannabis sativa. CNS Neurol Disord Drug Targets 2014;13:953‑960.

Crippa JAS, Derenusson GN, Ferrari TB, Wichert-Ana L, Duran FL, et al. Neural basis of anxiolytic effects of cannabidiol (CBD) in generalized social anxiety disorder: a preliminary report. J Psychopharmacol 2011;25:121‑130.

Moreira FA, Aguiar DC, Guimarães FS. Anxiolytic-like effect of cannabidiol in the rat Vogel conflict test. Prog Neuropsychopharmacol Biol Psychiatry 2006;30:1466‑1471.

Masataka N. Anxiolytic effects of repeated cannabidiol treatment in teenagers with social anxiety disorders. Front Psychol 2019;10:2466.

Almeida V, Levin R, Peres FF, Niigaki ST, Calzavara MB, et al. Cannabidiol exhibits anxiolytic but not antipsychotic property evaluated in the social interaction test. Prog Neuropsychopharmacol Biol Psychiatry 2013;41:30‑35.

Namdar D, Voet H, Ajjampura V, Nadarajan S, Mayzlish-Gati E, et al. Terpenoids and phytocannabinoids co-produced in Cannabis sativa strains show specific interaction for cell cytotoxic activity. Molecules 2019;24:3031.

Mammana S, Cavalli E, Gugliandolo A, Silvestro S, Pollastro F, et al. Could the combination of two non-psychotropic cannabinoids counteract neuroinflammation? Effectiveness of cannabidiol associated with cannabigerol. Medicina (Mex) 2019;55:747.

Scotter EL, Abood ME, Glass M. The endocannabinoid system as a target for the treatment of neurodegenerative disease. Br J Pharmacol 2010;160:480‑498.

Amin MR, Ali DW. Pharmacology of medical cannabis. Adv Exp Med Biol 2019;1162:151‑165.

Abdel-Salam OM, Youness ER, Khadrawy YA, Mohammed NA, Abdel-Rahman RF, et al. The effect of cannabis on oxidative stress and neurodegeneration induced by intrastriatal rotenone injection in rats. Comp Clin Pathol 2015;24:359‑378.

dos Reis Rosa Franco G, Smid S, Viegas C. Phytocannabinoids: general aspects and pharmacological potential in neurodegenerative diseases. Curr Neuropharmacol 2021;19:449‑464.

El-Remessy AB, Khalil IE, Matragoon S, Abou-Mohamed G, Tsai NJ, et al. Neuroprotective effect of (-) Δ9-tetrahydrocannabinol and cannabidiol in N-methyl-D-aspartate-induced retinal neurotoxicity: involvement of peroxynitrite. Am J Pathol 2003;163:1997‑2008.

El-Remessy AB, Al-Shabrawey M, Khalifa Y, Tsai NT, Caldwell RB, et al. Neuroprotective and blood-retinal barrier-preserving effects of cannabidiol in experimental diabetes. Am J Pathol 2006;168:235‑244.

Pickens JT. Sedative activity of cannabis in relation to its Δ ‘-trans-tetrahydrocannabinol and cannabidiol content. Br J Pharmacol 1981;72:649‑656.

Dutheil S, Ota KT, Wohleb ES, Rasmussen K, Duman RS. High-fat diet induced anxiety and anhedonia: impact on brain homeostasis and inflammation. Neuropsychopharmacology 2016;41:1874‑1887.

Lucas CJ, Galettis P, Schneider J. The pharmacokinetics and the pharmacodynamics of cannabinoids. Br J Clin Pharmacol 2018;84:2477‑2482.

Ohlsson A, Lindgren JE, Andersson S, Agurell S, Gillespie H, et al. Single-dose kinetics of deuterium-labelled cannabidiol in man after smoking and intravenous administration. Biomed Environ Mass Spectrom 1986;13:77‑83.

Newmeyer MN, Swortwood MJ, Barnes AJ, Abulseoud OA, Scheidweiler KB, et al. Free and glucuronide whole blood cannabinoids’ pharmacokinetics after controlled smoked, vaporized, and oral cannabis administration in frequent and occasional cannabis users: identification of recent cannabis intake. Clin Chem 2016;62:1579‑1592.

Toennes SW, Ramaekers JG, Theunissen EL, Moeller MR, Kauert GF. Comparison of cannabinoid pharmacokinetic properties in occasional and heavy users smoking a marijuana or placebo joint. J Anal Toxicol 2008;32:470‑477.

Grotenhermen F. Pharmacokinetics and pharmacodynamics of cannabinoids. Clin Pharmacokinet 2003;42:327‑360.

Dinis-Oliveira RJ. Metabolomics of Δ9-tetrahydrocannabinol: implications in toxicity. Drug Metab Rev 2016;48:80‑87.

Hunt CA, Jones RT. Tolerance and disposition of tetrahydrocannabinol in man. J Pharmacol Exp Ther 1980;215:35‑44.

Vickers NJ. Animal communication: when i’m calling you, will you answer too? Curr Biol 2017;27:R713‑R715.

Eichler M, Spinedi L, Unfer-Grauwiler S, Bodmer M, Surber C, et al. Heat exposure of Cannabis sativa extracts affects the pharmacokinetic and metabolic profile in healthy male subjects. Planta Med 2012;78:686‑91.

Chayasirisobhon S. Mechanisms of action and pharmacokinetics of cannabis. Perm J 2021;25.

Spindle TR, Cone EJ, Herrmann ES, Mitchell JM, Flegel R, et al. Pharmacokinetics of cannabis brownies: a controlled examination of Δ9-tetrahydrocannabinol and metabolites in blood and oral fluid of healthy adult males and females. J Anal Toxicol 2020;44:661‑671.

Grant KS, Petroff R, Isoherranen N, Stella N, Burbacher TM. Cannabis use during pregnancy: pharmacokinetics and effects on child development. Pharmacol Ther 2018;182:133‑151.

Belendiuk KA, Baldini LL, Bonn-Miller MO. Narrative review of the safety and efficacy of marijuana for the treatment of commonly state-approved medical and psychiatric disorders. Addict Sci Clin Pract 2015;10:1‑10.

Grotenhermen F, Kavanagh D, Zeit H. Scientific opinion on the safety of hemp (cannabis genus) for use as animal feed. EFSA J 2011;9:41.

Machado Bergamaschi M, Helena Costa Queiroz R, Waldo Zuardi A, Crippa AS. Safety and side effects of cannabidiol, a Cannabis sativa constituent. Curr Drug Saf 2011;6:237‑249.

Dryburgh LM, Bolan NS, Grof CP, Galettis P, Schneider J, et al. Cannabis contaminants: sources, distribution, human toxicity and pharmacologic effects. Br J Clin Pharmacol 2018;84:2468‑2476.

Wang GS, Post TW. Cannabis (marijuana): Acute intoxication. UpToDate Online 2019.

Fitzgerald KT, Bronstein AC, Newquist KL. Marijuana poisoning. Top Companion Anim Med 2013;28:8‑12.

Wong KU, Baum CR. Acute cannabis toxicity. Pediatr Emerg Care 2019;35:799‑804.

Sachs J, McGlade E, Yurgelun-Todd D. Safety and toxicology of cannabinoids. Neurotherapeutics 2015;12:735‑746.

Schoeler T, Bhattacharyya S. The effect of cannabis use on memory function: an update. Subst Abuse Rehabil 2013;4:11‑27.

Caulkins JP, Kilmer B, Kleiman MA. Marijuana legalization: What everyone needs to know®. Oxford University Press 2016.

Cohen PJ. Medical marijuana 2010: it’s time to fix the regulatory vacuum. J Law Med Ethics 2010;38:654‑666.

Kilmer B. Policy designs for cannabis legalization: starting with the eight Ps. Am J Drug Alcohol Abuse 2014;40:259‑261.

IssueVol 8, No 1, 2023 QRcode
SectionReview Article(s)
Cannabis sativa L. Botany Natural products Phytochemistry Phytopharmacology Traditional medicine

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How to Cite
Chaachouay N, Azeroual A, Bencharki B, Douira A, Zidane L. Cannabis sativa L.: A Review on Traditional Uses, Botany, Phytochemistry, and Pharmacological Aspects. Trad Integr Med. 2023;8(1):97-116.