Electrospun Poly Vinyl Alcohol Fiber Containing Lawsonia inermis L.: A Promising Effect on Burn Wound
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Abstract
Henna plant (Lawsonia inermis L.) has unique properties and has long been used as an herbal remedy. In this study, polyvinyl alcohol (PVA) nanofibers containing henna extract in two different concentrations were studied to prepare a wound dressing using electrospinning. PVA polymer nanofibers using henna extract with two different concentrations of 3% and 6% were prepared by the electrospinning method. Human fibroblasts were cultured on these fibers and the mechanical and survival properties of the cells, as well as the antibacterial properties of henna were compared and evaluated. The results showed that by using of henna extract in PVA nanofibers, cell viability was significantly reduced (P≤0.05). However, the ability of nanofibers to water uptake and mechanical properties of fibers significantly increased with increasing concentrations of henna extract (P≤0.05). Nanofiber wound dressing with antibacterial properties of henna extract provides relatively fast and rapid wound healing ability. The resulting fibers are encouraging candidates for the development of improved bandaging materials.
Boer M, Duchnik E, Maleszka R, Marchlewicz M. Structural and biophysical characteristics of human skin in maintaining proper epidermal barrier function. Adv Dermatol Allergol 2016;1:1-5.
Arda O, Göksügür N, Tüzün Y. Basic histological structure and functions of facial skin. Clin Dermatol 2014;32:3-13.
Hendriks FM, Brokken D, Oomens CWJ, Bader DL, Baaijens FPT. The relative contributions of different skin layers to the mechanical behavior of human skin in vivo using suction experiments. Med Eng Phys 2006;28:259-266.
Hettiaratchy S, Dziewulski P. Pathophysiology and types of burns. BMJ 2004;328:1427-1429.
Boateng J, Catanzano O. Advanced therapeutic dressings for effective wound healing- a review. J Pharm Sci 2015;104:3653-3680.
Warby R, Maani CV. Burn Classification. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021 [cited 2021 Sep 25]. Available from: http://www.ncbi.nlm.nih.gov/books/NBK539773/
Jeschke MG, van Baar ME, Choudhry MA, Chung KK, Gibran NS, et al. Burn injury. Nat Rev Dis Primer 2020;6:11.
Firenzuoli F, Gori L. Herbal medicine today: clinical and research issues. Evid-Based Complement Altern Med 2007;4:37-40.
Shamsi M, Abbasi N, Mohajer A, Hoseini M, Rafieian-Kopaei M. The most important native medicinal plants effective against cutaneous leishmaniasis in mouse. Int J Life Sci Pharma Res 2018;8:7.
Eisenberg DM, Davis RB, Ettner SL, Appel S, Wilkey S, et al. Trends in alternative medicine use in the united states, 1990-1997: results of a follow-up national survey. JAMA 1998;280:1569-1575.
Elzayat EM, Auda SH, Alanazi FK, Al-Agamy MH. Evaluation of wound healing activity of henna, pomegranate and myrrh herbal ointment blend. Saudi Pharm J. 2018;26:733-738.
Goyal BR, Goyal RK, Mehta AA. Phyto-pharmacology of achyranthes aspera: a review. Pharmacogn Rev 2007;1:143-150.
Biswas K, Chattopadhyay I, Banerjee RK, Bandyopadhyay U. Biological activities and medicinal properties of Neem (Azadirachta indica). Curr Sci 2002;82:1336-1345.
Pasandi Pour A, Farahbakhsh H. Lawsonia inermis L. leaves aqueous extract as a natural antioxidant and antibacterial product. Nat Prod Res 2020;34:3399-3403.
Badoni Semwal R, Semwal DK, Combrinck S, Cartwright-Jones C, Viljoen A. Lawsonia inermis L. (henna): Ethnobotanical, phytochemical and pharmacological aspects. J Ethnopharmacol 2014;155:80-103.
Al-Rubiay KK, Jaber NN, Al-Mhaawe BH, Alrubaiy LK. Antimicrobial efficacy of henna extracts. Oman Med J 2008;23:253-256.
Mikhaeil BR, Badria FA, Maatooq GT, Amer MMA. Antioxidant and immunomodulatory constituents of henna leaves. Z Für Naturforschung C. 2004;59:468-476.
Singh Malik D, Mital N, Kaur G. Topical drug delivery systems: a patent review. Expert Opin Ther Pat 2016;26:213-228.
Gaaz T, Sulong A, Akhtar M, Kadhum A, Mohamad A, et al. Properties and applications of polyvinyl alcohol, halloysite nanotubes and their nanocomposites. Molecules 2015;20:22833-22847.
Groeber F, Holeiter M, Hampel M, Hinderer S, Schenke-Layland K. Skin tissue engineering - in vivo and in vitro applications. Adv Drug Deliv Rev 2011;63:352-366.
Restrepo I, Medina C, Meruane V, Akbari-Fakhrabadi A, Flores P, et al. The effect of molecular weight and hydrolysis degree of poly (vinyl alcohol) (PVA) on the thermal and mechanical properties of poly(lactic acid)/PVA blends. Polímeros 2018;28:169-177.
Mirjalili M, Zohoori S. Review for application of electrospinning and electrospun nanofibers technology in textile industry. J Nanostructure Chem 2016;6:207-213.
Xue J, Wu T, Dai Y, Xia Y. Electrospinning and electrospun nanofibers: methods, materials, and applications. Chem Rev 2019;119:5298-5415.
Opanasopit P, Ruktanonchai U, Suwantong O, Panomsuk S, Ngawhirunpat T, et al. Electrospun poly(vinyl alcohol) fiber mats as carriers for extracts from the fruit hull of mangosteen. J Cosmet Sci 2008;59:233-242.
Vahabi S, Hakemi-Vala M, Gholami S. In vitro antibacterial effect of hydroalcoholic extract of lawsonia inermis, malva sylvestris, and boswellia serrata on aggregatibacter actinomycetemcomitans. Adv Biomed Res 2019;8:22.
Zahedi P, Rezaeian I, Ranaei-Siadat S-O, Jafari S-H, Supaphol P. A review on wound dressings with an emphasis on electrospun nanofibrous polymeric bandages: review on wound dressings. Polym Adv Technol 2010;21:77-95.
Yarin AL, Koombhongse S, Reneker DH. Bending instability in electrospinning of nanofibers. J Appl Phys 2001;89:3018-3026.
Kumar PTS, Praveen G, Raj M, Chennazhi KP, Jayakumar R. Flexible, micro-porous chitosan-gelatin hydrogel/nanofibrin composite bandages for treating burn wounds. RSC Adv 2014;4:65081-65087.
Ma PX, Zhang R. Synthetic nano-scale fibrous extracellular matrix. J Biomed Mater Res 1999;46:60-72.
Zumrutdal E, Ozaslan M. A miracle plant for the herbal pharmacy; henna (lawsonia inermis). Int J Pharmacol 2012;8:483-489.
Ray SS, Chen S-S, Li C-W, Nguyen NC, Nguyen HT. A comprehensive review: electrospinning technique for fabrication and surface modification of membranes for water treatment application. RSC Adv 2016;6:85495-85514.
Sadri M, Arab-Sorkhi S, Vatani H, Bagheri-Pebdeni A. New wound dressing polymeric nanofiber containing green tea extract prepared by electrospinning method. Fibers Polym 2015;16:1742-1750.
Ranjbar-Mohammadi M, Bahrami SH. Development of nanofibrous scaffolds containing gum tragacanth/poly (ε-caprolactone) for application as skin scaffolds. Mater Sci Eng C 2015;48:71-79.
Gomes SR, Rodrigues G, Martins GG, Roberto MA, Mafra M, et al. In vitro and in vivo evaluation of electrospun nanofibers of PCL, chitosan and gelatin: a comparative study. Mater Sci Eng C 2015;46:348-358.
Li Q, Gao W-Q, Zhao Y-Q. Advances in studies on chemical constituents and biological activities of Lawsonia inermis. Zhongguo Zhong Yao Za Zhi 2013;38:795-799.
Malekzadeh F. Antimicrobial activity of Lawsonia inermis L. Appl Microbiol 1968;16:663-664.
Jridi M, Sellimi S, Lassoued KB, Beltaief S, Souissi N, et al. Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract. Colloids Surf Physicochem Eng Asp 2017;512:71-79.
Kowalczyk MC, Walaszek Z, Kowalczyk P, Kinjo T, Hanausek M, et al. Differential effects of several phytochemicals and their derivatives on murine keratinocytes in vitro and in vivo: implications for skin cancer prevention. Carcinogenesis 2009;30:1008-1015.
Menazea AA, Ahmed MK. Wound healing activity of chitosan/polyvinyl alcohol embedded by gold nanoparticles prepared by nanosecond laser ablation. J Mol Struct 2020;1217:128401.
Dodero A, Alloisio M, Castellano M, Vicini S. Multilayer alginate–polycaprolactone electrospun membranes as skin wound patches with drug delivery abilities. ACS Appl Mater Interfaces 2020;12:31162-31171.
Mohammed Rahmoun N, Boucherit-Atmani Z, Benabdallah M, Boucherit K, Villemin D, et al. Antimicrobial activities of the henna extract and some synthetic naphthoquinones derivatives. Am J Med Biol Res 2013;1:16-22.
Yousefi I, Pakravan M, Rahimi H, Bahador A, Farshadzadeh Z, et al. An investigation of electrospun Henna leaves extract-loaded chitosan based nanofibrous mats for skin tissue engineering. Mater Sci Eng C 2017;75:433-444.
| Files | ||
| Issue | Vol 7, No 3, 2022 | |
| Section | Research Article(s) | |
| DOI | https://doi.org/10.18502/tim.v7i3.10767 | |
| Keywords | ||
| Poly Vinyl Alcohol L. inermis Wound dressing Burn Electrospinning | ||
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