Investigation of in Vitro Wound Healing Activity of Polygonatum orientale Desf. Rhizome
Abstract
In this study, we carried out a bioassay-guided fractionation of the most active extract Polygonatum orientale Desf. rhizome, in order to isolate and identify the fraction/s or compound/s responsible for wound healing activity. The wound healing process considered via scratch wound assay on NIH-3T3 fibroblasts. The results showed that the methanol extract and its fractions A5 and A6 showed excellent wound healing effect and were rich of bioactive glycoside compounds. Fraction A6 was selected for further fractionation and two sub-fractions B5 and B6 showed acceptable wound healing on fibroblasts. B5-P (sucrose) and B6-P were isolated as two active compounds from theses fractions that significantly reduced wound area, without any toxicity at very low concentrations (50-200 ng/mL). These results supported the traditional use of P. orientale rhizome for wounds treatment and showed that the accelerated wound healing activity of the rhizome is due to the presence of bioactive polar compounds.
Zargari A. The Medicinal Plants (in Persian). Tehran, Tehran University. 1990;4: 702-722.
Mozaffarian, V. Recognition of Iranian Medicinal and Aromatic Plants: Contemporary Culture. 2012; pp 631-632.
Amin G. The Most Common Traditional Medicinal Plants of Iran. Tehran University of Medical Sciences and Health Services. Tehran 2004; p 190.
Khorasani, MHA. Makhzan ol Advieh (Drugs Resevoir), Tehran University of Medical Sciences Press. Tehran 2009.
Sadati Lamardi SN, Ghambariyan S, Shams Ardekani MR, Amin G. Major phytosteroid from Polygonatum orientale Desf. Rhizome. Int J Med Res Health Sci 2017;5:43-49.
Zhao P, Zhao C, Li X, Gao Q, Huang L, et al. The genus Polygonatum: A review of ethnopharmacology, phytochemistry and pharmacology. J Ethnopharmacol 2018;214:274-291.
Fathi FM, Harita H, Mahmood AA, Suzy SM, Salmah I, et al. Acceleration of wound healing activity by Polygonatum odoratum leaf extract in rats. J Med Plants Res 2014;8:523-528.
Yeşlada E, Houghto PJ. Steroidal saponins from the rhizomes of Polygonatum orientale. Phytochem 1991;30:3405-3409.
Bainbridge P. Wound healing and the role of fibroblasts. J. Wound Care 2013;22: 407-412.
Nagori BP, Solanki R. Role of medicinal plants in wound healing. Res J Med Plant 2011;5:392-405.
Moulin V, Castilloux G, Jean A, Garrel DR, Auger FA, et al. In vitro models to study wound healing fibroblasts. Burns 1996;22:359-362.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
Sarkhail P. Navidpour L, Rahimifard M, Hosseini N, Souri E. Bioassay-guided fractionation and identification of wound healing active compound from Pistacia vera L. hull extract. J Ethnopharmacol 2019;248:112335.
Savic V, Nikolic V, Arsic I, Stanojevic L, Najman S, et al. Comparative study of the biological activity of allantoin and aqueous extract of the comfrey root. Phyther Res 2015;29:1117-1122.
Wenzler M, Hölscher D, Oerther T, Schneider B. Nectar formation and floral nectary anatomy of Anigozanthos flavidus: A combined magnetic resonance imaging and spectroscopy study. J Exp Bot 2008;59:3425-3434.
Koopman J, Grimme S. Calculation of electron ionization mass spectra with semiempirical GFNn-xTB Methods. ACS Omega 2019;4:15120-1533.
Calvano CD, Cataldi TRI, Kögel JF, Monopoli A, Palmisano F, et al. Structural characterization of neutral saccharides by negative ion MALDI mass spectrometry using a superbasic proton sponge as deprotonating matrix. J Am Soc Mass Spectrom 2017;28:1666-1675.
Beckett ST, Francesconi MG, Geary PM, Mackenzie G, Maulny APE. DSC study of sucrose melting. Carbohydr Res 2006;341:2591-2599.
Liang CC, Park AY, Guan JL. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2007;2:329-333.
Cory G. Scratch-wound assay. Meth Mol Biol 2011;769:25-30.
Bibi Y, Arshad M, Sabir S, Amjad MS, Ahmed E, et al. Pharmacology and biochemistry of Polygonatum verticillatum: A review. J Coast Life Med 2016;4: 406-415.
Nakao H, Yamazaki M, Tsuboi R, Ogawa H. Mixture of sugar and povidone - Iodine stimulates wound healing by activating keratinocytes and fibroblast functions. Arch Dermatol Res 2006;298:175-182.
Ali F, Monica WS, Adikurniawan YM, Sari DK, Amir MN. Fibroblast cell description of provision of sugar and honey in incision wound of domestic cat (Felis domestica). IOP Conf Ser Earth Environ Sci 2020;575:012027.
Topham J. Sugar for wounds. J Tissue Viability 2000;10:86-89.
Fu YL, Yu ZY, Tang XM, Zhao Y, Yuan XL, et al. Pennogenin glycosides with a spirostanol structure are strong platelet agonists: Structural requirement for activity and mode of platelet agonist synergism. J Thromb Haemost 2008;6:524-533.
Yu HS, Ma BP, Kang LP, Zhang T, Jiang FJ, et al. Saponins from the processed rhizomes of Polygonatum kingianum. Chem Pharm Bull 2009;57:1011-1014.
Kim YS, Cho IH, Jeong MJ, Jeong SJ, Nah SY, et al. Therapeutic effect of total ginseng saponin on skin wound healing. J Ginseng Res 2011;35:360-367.
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| Issue | Vol 7, No 2, 2022 | |
| Section | Research Article(s) | |
| DOI | https://doi.org/10.18502/tim.v7i2.9925 | |
| Keywords | ||
| Polygonatum orientale rhizome Fibroblasts 3-(4,5-dimethylthiazol-2-yl) 2 5-diphenyltetrazolium bromide (MTT) assay Scratch wound healing assay Sucrose | ||
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