Tehran University of Medical Sciences Traditional and Integrative Medicine 2476-5104 7 2 2022 06 20 231 243 Ghazal Hashemi Department of Pharmacognosy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Hamid-Reza Adhami Department of Pharmacognosy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Mahban Rahimifard Toxicology and Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran AND Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Maryam Baeeri Toxicology and Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran AND Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Parisa Sarkhail Medicinal Plants Research Center, School of Pharmacy, Tehran University of Medical Sciences. Tehran, Iran 2021 08 26 2021 10 28 In this study, we carried out a bioassay-guided fractionation of the most active extract Polygonatum orientale Desf. rhizome, in order to isolate and identify the fraction/s or compound/s responsible for wound healing activity. The wound healing process considered via scratch wound assay on NIH-3T3 fibroblasts. The results showed that the methanol extract and its fractions A5 and A6 showed excellent wound healing effect and were rich of bioactive glycoside compounds. Fraction A6 was selected for further fractionation and two sub-fractions B5 and B6 showed acceptable wound healing on fibroblasts. B5-P (sucrose) and B6-P were isolated as two active compounds from theses fractions that significantly reduced wound area, without any toxicity at very low concentrations (50-200 ng/mL). These results supported the traditional use of P. orientale rhizome for wounds treatment and showed that the accelerated wound healing activity of the rhizome is due to the presence of bioactive polar compounds. https://jtim.tums.ac.ir/index.php/jtim/article/view/524