Extraction and Identification of Chemical Compounds of Peganum harmala L. Seed Essential Oil by HS-SPME and GC-MS Methods
Abstract
Peganum harmala is a plant that belongs to the family Zygophyllaceae. In traditional medicine, this plant has therapeutic effects such as repelling intestinal worms, increasing sexual potency, increasing milk secretion, anti-rheumatism, regularizing, anti-parasitic, hypnotic, diaphoretic, anti-cancer and analgesic. The aim of this study was to identify the chemical composition of P. harmala seed essential oil. P. harmala seed essential oil was extracted by Head Space-SPME [HS-SPME] method and its chemical compounds were identified by GC-MS method. Totally, 41 chemical compounds were identified in P. harmala seed essential oil. According to the results, the most compounds of P. harmala L. seed essential oil includes 2,3-dimethyl benzofuran (28.32%), cis-linalool oxide (7.46%), [2E]- decenal (6.57%), 4aα,7β,7aα-nepetalactone (6.49%), 3-oxo-p-menth-1-en-7-al (6.36%) and trans- β- terpineol (5.86%), respectively.
Asgarpanah, J. Ramezanloo F. Chemistry, pharmacology and medicinal properties of Peganum harmala L. J Afr Pharma Pharmacol 2012;6:1573-1580.
Sobhani AM, Ebrahimi SA, Mahmoudian M. An in vitro evaluation of human DNA topoisomerase I inhibition by Peganum harmala L. seeds extract and its beta-carboline alkaloids. J Pharm Pharm Sci 2002;5:19-23.
Cordell GA. pharmacology of Ibogaine and Ibogainerelated alkaloids. The Alkaloids 1998; 52:197-231.
Ribaa J, Andererb P, Janéa F, Saletub B, Barbanoja MJ. Effects of the south american psychoactive beverage ayahuasca on regional brain electrical activity in humans: A functional neuroimaging study using low-resolution electromagnetic tomography. Neuropsychobiology 2004;50;84-101.
Al-allaf TA, Khizaie RF, Rashan LJ, Halaseh WF. Cytotoxicity activity of a series of tumor cell lines with various tumor ligands. Boll Chim Farm 1999;138:267-271.
Sharbatkori M. Study of cidal effect of alcoholic extract of Peganum harmala seeds on Echinococcus granulosus protoscolex. [Dissertation] Tehran University of Medical Sciences, Iran; 2007.
Lord H, Pawliszyn J. Microextraction of drugs. J Chromatogr A 2000;902:17-63.
Hashemi P, Yarahmadi A, Shamizadeh M, Khademi K. Comparison of headspace solvent microextraction, hydrodistillation solvent microextraction, and solid-phase microextraction for the study of volatile components of Kelussia odoratissima Mozaff. by GCMS. Acta Chromatogr 2012;24:97-109.
Bahmani M, Taherikalani M, Khaksarian M, Soroush S, Ashrafi B et al. Phytochemical Profiles and Antibacterial Activities of Hydroalcoholic Extracts of Origanum vulgare and Hypericum perforatum and Carvacrol and Hypericin as a Promising Anti-Staphylococcus aureus. Mini Rev Med Chem 2019;19:923-932.
Sassoui D, Seridi R, Azin K, Usai M. Evaluation of phytochemical constituents by GC-MS and antidepressant activity of Peganum harmala L. seeds extract. Asian Pac J Trop Dis 2015;5:971-974.
Apostolico I, Aliberti L, Caputo L, De Feo V, Fratianni F et al. Chemical Composition, Antibacterial and Phytotoxic Activities of Peganum harmala Seed Essential Oils from Five Different Localities in Northern Africa. Molecules 2016;21:1235.
Nicholas CD, Sanders-Bush E. Serotonin receptor signaling and hallucinogenic drug action. Heffter Rev Psycholeptic Res 2001;2:73-79.
Abdel-Fattah AFM, Matsumoto K, Gammaz HAK, Watanabe H. Hypothermic effect of harmala alkaloid in rats: involvement of serotonergic mechanism. Pharmacol Biochem Behav 1995;52:421-426.
Adell A, Biggs T, Myers R. Action of harman (1-methyl-β-carboline) on the brain: Body temperature and in vivo efflux of 5-HT from hippocampus of the rat. Neuropharmacology 1996;35:1101-1107.
Hillberp C, Hapillon P. Effect of harmaline on anxiety related behavior in mice. Physiol Behav 2005; 86: 164- 167.
Loub W, Farnsworth N, Soejarto D, Quinn M. NAPRALERT: computer handling of natural product research data. J Chem Inf Comput Sci 1985;25:99-103.
Glasby J. Encyclopedia of the alkaloids 1978;3:658-661.
Herraiz T, Gonzalez D, Ancin-Apilicueta C, Aran VJ, Guillen H. Beta-Carboline alkaloids in Pegannum harmala and inhibition of human monoamine oxidase (MAO). Food Chem Toxicol 2010;48:839-845.
Nenaah G. Antibacterial and antifungal activities of (beta)-carboline alkaloids of Peganum harmala (L) seeds and their combination effects. Fitoterapia 2010;81:779-782.
Yannai Sh. Dictionary of food compounds with CD-ROM: Additives, flavors, and ingredients. Boca Raton: Chapman & Hall/CRC 2014.
Bahmani M, Hadavi M, Abbasi N. Study of extraction and chemical compounds of Scrophularia striata Boiss. and Scrophularia deserti Delile using HS-SPME and GC-MS. Plant Biotechnol Persa 2020;2:8-13.
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Issue | Vol 6, No 3, 2021 | |
Section | Research Article(s) | |
DOI | https://doi.org/10.18502/tim.v6i3.7310 | |
Keywords | ||
Medicinal plant Essential oil Peganum harmala L. 2,3-Dimethyl benzofuran Headspace solid-phase microextraction (HS-SPME) Gas chromatography mass spectrometry (GC-MS) |
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