Protective Effect of Vitis gracilis Wall (Vitaceae) Leaf Decoction on Sexual Vitality and Testis of Alloxan-Induced Diabetic Mice
,
Abstract
Diabetes mellitus is profoundly associated with various detrimental outcomes including sexual dysfunction and infertility in males. On the other hand, a medicinal plant namely Vitis gracilis Wall (Vitaceae) has been used as a traditional medicine to enhance vitality. This present study aimed to investigate the protective effect of V. gracilis leaf decoction against diabetes-induced sexual dysfunction and testicle histopathology in adult male mice. The experiment was composed of five different groups namely the control (non-diabetic) group, the diabetes group (without any treatments), and the diabetes treated with V.gracilis decoction at the doses of 25, 50, and 100 g/ L, respectively. In addition, the phytochemical constituents of leaf decoction were determined by using Ultra performance-liquid chromatography-mass spectroscopy (UPLC-MS). Our data demonstrated that, despite failing to improve blood glucose profile and body weight, V.gracilis leaf decoction sustained intense sexual behaviors including face and genital kissing, genital licking, and mount toward estrous females. Moreover, lower doses of decoction (25 and 50 g/ L) attenuated the diabetes-induced reduction of testis weight and precluded malondialdehyde accumulation in the testicle tissue. The decoction at the lower doses also ameliorated histopathological alterations in the testis, particularly the wall thickness of tubulus seminiferous and the number of necrotic cells. V. gracilis decoction also improved hematological values including hemoglobin (Hb), red blood cell count and hematocrit (HTC) level. In addition, UPLC-MS analysis revealed a total of 26 phytochemical compounds with seven predominant substances. In conclusion, leaf decoction of V. gracilis, particularly at lower doses but not at a higher dose, exerted a protective effect on sexual vitality, testicle tissue, and hematological value under diabetic condition. The beneficial effects of V.gracilis decoction might be associated with its various bioactive compounds. Therefore, V. gracilis leaves may be a future candidate as a potent natural drug for male sexual vitality and testicle protection against diabetes.
Shi GJ, Zheng J, Wu J, Qiao HQ, Chang Q, et al. Protective effects of Lycium barbarum polysaccharide on male sexual dysfunction and fertility impairments by activating hypothalamic pituitary gonadal axis in streptozotocin-induced type-1 diabetic male mice. Endocr J 2017;64:907-922.
Zeleke M, Hailu D, Daka D. Erectile dysfunction and associated factors among diabetic patients at, Hawassa, Southern, Ethiopia. BMC Endocr Disord 2021;21:139.
Fan J, Peng T, Hui J, Ding W, He B, et al. Erectile Dysfunction in Type-2 diabetes mellitus patients: predictors of early detection and treatment. Urol Int 2021;5:986-992.
Jacobson AM, Braffett BH, Cleary PA, Dunn RL, Larkin ME, et al. Relationship of urologic complications with health-related quality of life and perceived value of health in men and women with type I diabetes: the Diabetes Control and Complications Trial/Epidemiology of Interventions and Complications (DCCT/EDIC) cohort. Diabetes Care 2015;38:1904–1912.
Bracchi M, Stuart D, Castles R, Khoo S, Back D, et al. Increasing use of party drugs in people living with HIV on antiretrovirals: a concern for patient safety. AIDS 2015;29:1585-1592.
Derosa G, D'Angelo A, Preti PS, Maffioli P. Evaluation of the effect on sexual performance of a nutraceutical combination containing alpha lipoic acid, Vitis vinifera L. and Ginkgo biloba, compared to placebo, avanafil or a combination of nutraceutical plus avanafil in males with type 2 diabetes mellitus with erectile dysfunction. Front Endocrinol 2022;13:847240.
Duangjan C, Rangsinth P, Zhang S, Gu X, Wink M, et al. Vitis Vinifera leaf extract protects against glutamate-induced oxidative toxicity in HT22 hippocampal neuronal cells and increases stress resistance properties in caenorhabditis elegans. Front Nutr 2021;8:634100.
Marabini L, Melzi G, Lolli F, Dell'Agli M, Piazza S, et al. Effects of Vitis vinifera L. leaves extract on UV radiation damage in human keratinocytes (HaCaT). J Photochem Photobiol B 2020;204:111810.
Meng L, Jiao Y, Zhou X, Liang C, Yan K, et al . Leaf extract from Vitis vinifera L. reduces high fat diet-induced obesity in mice. Food Funct 2021;12:6452-6463.
Lin SY, Huang GC, Hsieh YY, Lin YS, Han CH, et al. Vitis thunbergii var. taiwaniana extracts and purified compounds ameliorate obesity in high-fat diet-induced obese mice. J Agric Food Chem 2015;63:9286-9294.
Lu YL, Lin SY, Fang SU, Hsieh YY, Chen CR, et al. Hot-water extracts from roots of Vitis thunbergii var. taiwaniana and identified ε-Viniferin improve obesity in high-fat diet-induced mice. J Agric Food Chem 2017;65:2521-2529.
Aththorick TA, Berutu L. Ethnobotanical study and phytochemical screening of medicinal plants on Karonese people from North Sumatra, Indonesia. J Phys Conf Ser 2018;116:052008.
Wasnis NZ, Ilyas S, Hutahaean R, Silaban R, Situmorang PC. Effect of Vitis gracilis Wall (gagatan harimau) in the recovery of gastrocnemius muscle cells and cytochrome c expression of Mus musculus. J Pharm Pharmacogn Res 2022;10:303-309.
Marouani N, Chahed A, Hédhili A, Hamdaoui MH. Both aluminum and polyphenols in green tea decoction (Camellia sinensis) affect iron status and hematological parameters in rats. Eur J Nutr 2007;46:453-459.
Yin P, Wang Y, Yang L, Sui J, Liu Y. Hypoglycemic effects in alloxan-induced diabetic rats of the phenolic extract from mongolian oak cups enriched in ellagic acid, kaempferol and their derivatives. Molecules 2018;23:1046.
Hichor M, Sundaram VK, Eid SA. Liver X receptor exerts a protective effect against the oxidative stress in the peripheral nerve. Sci Rep 2018;8:2524.
Yao L, Wei J, Shi S, Guo K, Wang X, et al. Modified lingguizhugan decoction incorporated with dietary restriction and exercise ameliorates hyperglycemia, hyperlipidemia and hypertension in a rat model of the metabolic syndrome. BMC Complement Altern Med 2017;17:132.
Rew KT. Men's health: male sexual dysfunction. FP Essent 2021;503:28-33.
Minaz N, Razdan R, Hammock BD, Mujwar S, Goswami SK. Impact of diabetes on male sexual function in streptozotocin-induced diabetic rats: Protective role of soluble epoxide hydrolase inhibitor. Biomed Pharmacother 2019;115:108897.
Wagner IV, Klöting N, Savchuk I, Eifler L, Kulle A, et al. Diabetes type 1 negatively influences leydig cell function in rats, which is partially reversible by insulin treatment. Endocrinology 2021;162:bqab017.
Lamsaard S, Sukhorum W, Arun S, Phunchago N, Uabundit N, et al. Valproic acid induces histologic changes and decreases androgen receptor levels of testis and epididymis in rats. Int J Reprod Biomed 2017;15:217-224.
Asmat U, Abad K, Ismail K. Diabetes mellitus and oxidative stress-A concise review. Saudi Pharm J 2016;24:547-553.
Nna VU, Abu BAB, Ahmad A, Eleazu CO, Mohamed M. Oxidative stress, NF-κB-mediated inflammation and apoptosis in the testes of streptozotocin-induced diabetic rats: combined protective effects of malaysian propolis and metformin. Antioxidants (Basel) 2019; 8:465.
Takebayashi J, Ishii R, Chen J, Matsumoto T, Ishimi Y, et al. Reassessment of antioxidant activity of arbutin: multifaceted evaluation using five antioxidant assay systems. Free Radic Res 2010;44:473-478.
Alex EA, Tanko Y, Muhammed K, Dubo AB, Yahuza FM, et al. Modulatory role of lauric acid supplement on lipid peroxidation and some antioxidant enzymes activity in high fat diet, streptozotocin-induced type 2 diabetic male wistar rats. J Afr Ass Physiol Sci 2019;7:23-29.
Wang ZS, Song ZC, Bai JH. Red blood cell count as an indicator of microvascular complications in Chinese patients with type 2 diabetes mellitus. Vasc Health Risk Manag 2013;9:237-243.
Wang Y, Yang P, Yan Z, Liu Z, Ma Q, et al. The Relationship between Erythrocytes and Diabetes Mellitus. J Diabetes Res 2021;2021:6656062.
Taderegew MM, Gebremariam T, Tareke AA, Woldeamanuel GG. Anemia and its associated factors among type 2 diabetes mellitus patients attending debre berhan referral hospital, north-east ethiopia: a cross-sectional study. J Blood Med 2020;11:47-58.
Wasnis NZ, Ilyas S, Hutahaean S, Silaban R, Situmorang PC. Analysis of apoptotic cells and lung inflammation after given by Vitis gracilis. Pak J Biol Sci 2022;25:1033-1039.
| Files | ||
| Issue | Vol 8, No 3, 2023 | |
| Section | Research Article(s) | |
| DOI | https://doi.org/10.18502/tim.v8i3.13711 | |
| Keywords | ||
| Diabetes mellitus Male fertility Malondialdehyde Sexual dysfunction Vitaceae | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
