Antibacterial Activity of Aerial Part Extracts and Fractions of Ajuga chamaecistus ssp. tomentella
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Abstract
The genus Ajuga [Kamafitos] has been used as wound healing in traditional Persian medicine. Recent studies have shown that some species of this plant has antibacterial effects. Methanol 80% and aqueous extract and partition fractions of n-hexane, diethyl ether, and n-butanolic from a methanolic extract of Ajuga chamaecistus ssp. tomentella were evaluated for antibacterial activities against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa using cup-plate method. Furthermore, (minimum inhibitory concentration) MIC was determined using micro plate method. Gentamicin and vancomycin were used as positive control and methanol 5% was used as negative control. Zone of inhibition (mm) and the MIC (µg/ml) were measured in three times. Based on the results, all extracts and fractions showed antibacterial effect against S. aureus, B. subtilis, E. coli and P. aeruginosa, among them the diethyl ether fraction exhibited the most antibacterial effect against S. aureus (zone of inhibition =18mm) and B. subtilis (zone of inhibition =16.5mm). Also, n-butanolic fraction showed the most antibacterial effect against E. coli (zone of inhibition =16mm) and methanolic extract presented the most antibacterial effect against B. subtilis (zone of inhibition =15mm). S. aureus was the most sensitive (MIC>333µg/ml) and P. aeruginosa was the most resistant (MIC>4000µg/ml) bacteria to the extracts. The above results revealed that most of the extracts and fractions from this plant possess antibacterial activity against micro-organisms specially S. aureus. According to traditional use of Ajuga species as wound healing medicinal plant, it can be used for treatment of various skin infections and possibly reduction of healing time.
Chandra H, Bishnoi P, Yadav A, Patni B, Mishra AP, Nautiyal AR. Antimicrobial resistance and the alternative resources with special emphasis on plant-based antimicrobials-a review. Plants 2017;6:16.
Aghili Khorasani MH. Drugs Resevoir (Makhzan ol Advieh). Tehran University of Medical Sciences Press. Tehran 2009 [In Persian].
Naghibi F, Mosaddegh M, Mohammadi Motamed S, Ghorbani A. Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology. Iran J Pharm Res 2005;2:63-79.
Mozaffarian V. A Dictionary of Iranian Plant Names. 5th ed. Farhang moaser Press. Tehran 2007; pp 21-22 [In Persian].
Ono Y, Fukaya Y, Imai S, Yamakuni T. Beneficial effects of Ajuga decumbens on osteoprosis and arthritis. Biol Pharm Bull 2008;31:1199-1204.
Gautam R, Jachak SM, Saklani A: Anti-inflammatory effect of Ajuga bracteosa Wall ex Benth. mediated through cyclooxygenase (COX) inhibition. J Ethnophar 2011;133:928-930.
Hilaly JE, Lyoussi B. Hypoglycemic effect of lyophilized total water extract of Ajuga iva in normal and streptozotocin diabetic rats. J Ethnophar 2002;80:109-113.
Kuria KAM, Coster S, Muriuki G, Masengo W, Kibwage I, Hoogmartens J, Laekeman GM. Antimalarial activity of Ajuga remota Benth (labiatae) and Caesalpinia volkensii Harms (Caesalpiniaceae): in vitro confirmation of ethnopharmacological use. J Ethnophar 2001;74:141-148.
Mamadalieva NZ, El-Ready MZ, Ovidi. Antiproliferative, antimicrobial and antioxidant activities of chemical constituents of Ajuga turkestanica. Phytopharmacology 2013;4:1-18.
Shams Ardekani MR, Khanavi M, Taheri P, Samadi N, Safaripour E, Salimpour F: The essential oil composition of Ajuga chamaecistus Ging. subsp. tomentella Rech. f. JEOBP 2010;13:45-51.
Sadati N, Ostad S N, Karimian Z, Shams Ardekani M R, Akbarzadeh T, Hadjiakhoondi A, Khanavi M: Phytochemical study and in vitro cytotoxic effect of Ajuga chamaecistus ssp. tomentella. Asian J Chem 2012;24:2871-2874.
Sadati N, Jenett-Siems K, Siems K, Shams Ardekani MR, Hadjiakhoondi A, Akbarzadeh T, Ostad SN, Khanavi M: Major constituents and cytotoxic effects of Ajuga chamaecistus subsp. tomentella. Z Naturforsch 2012;67:275-281.
Khanavi M, Davoodipoor AM, Sadati SN, Shams Ardekani MR, Sharifzadeh M. Antinociceptive effect of some extracts from Ajuga chamaecistus Ging. ssp. tomentella (Boiss.) Rech. f. aerial parts. DARU 2014;22:56.
Khanavi M, Najafi B, Sadati SN, Abai MR, Vatandoost H. Chemical Constitute and Larvicidal activity of fractions of Ajuga chamaecistus tomentella plant against malaria vector Anopheles stephensi. J Arthropod-Borne Dis 2017;11:116-123.
Fazly-Bazzaz BS, Khajehkaramadin M, Shokooheizadeh HR. Antibacterial activity of Rheum ribes extract obtained from various plant parts against clinical isolates of Gram-negative pathogens. Iran J Pharm Res 2005;2:87-91.
European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID). Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by broth dilution. Clin Microb infect 2003;9:1-7.
Ayari B, Riahi L, Ziadi S, Chograni H, Mliki A. Evaluation of antioxidant and antimicrobial activities of Tunisian Ajuga iva L. Essential oils. Revue F S B XI 2013;203-210.
Mothana RA, Alsaid MS, Hasoon SS, Al-Mosaiyb NM, Al-Rehaily AJ, Al-Yahya MA. Antimicrobial and antioxidant activities and gas chromatography mass spectrometry (GC/MS) analysis of the essential oils of Ajuga bracteosa Wall. ex Benth. and Lavandula dentata L. growing wild in Yemen. J Med Plants Res 2012;6:3066-3071.
Rahman N, Ahmad M, Riaz M, Mehjabeen, Jahan N, Ahmad R. Phytochemical, antimicrobial, insecticidal and brine shrimp lethality bioassay of the crude methanolic extract of Ajuga parviflora Benth. Pak J Pharm Sci 2013;26:751-756.
Chaari A, Jannet HB and Mighri Z. Isolation and structural determination of a novel antibacterial phytoecdysteroid and of two cinnamic acid derivatives based on Ajuga pseudoiva Leaves. J Soc Chim 2000;4:789-799.
Mamadalieva NZ, El-Read MZ, Ovidi E, Ashou ML, Hamoud R, Sagdullaev SS. Antiproliferative, antimicrobial and antioxidant activities of the chemical constituents of Ajuga turkestanica. Phytopharmacology 2013;4:1-18.
Ganaie HA, Ali MN, Ganai BA, Meraj M, Ahmad M. Antibacterial activity of 14, 15 -dihydroajugapitin and 8-o-acetylharpagide isolated from Ajuga bracteosa Wall ex. Benth against human pathogenic bacteria. Microb Pathog 2017;103:114-118.
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| Issue | Vol 2, No 2, 2017 | |
| Section | Research Article(s) | |
| Keywords | ||
| Ajuga chamaecistus subsp tomentella Antibacterial Cup-plate method MIC | ||
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