Antioxidant Activity of Lallemantia royleana (Benth.) Seed Extract

  • Mahbubeh Bozorgi Department of Traditional Pharmacy, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Mahdi Vazirian Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Lallemantia royleana, balangu, Antioxidant activity, Persian medicine


Lallemantia royleana seeds have been used in Persian traditional medicine during the ages. The seeds are known as “Balngu” in Iran and still are widely used as an ethnomedicine for treatment purposes such as in gastrointestinal disease, kidney and urinary disorders and skin complications. In this study,antioxidant and total phenolic content of L. royleana seeds were investigated. Seeds of L. royleana (50 g) were crushed using a laboratory mill. Ground material (50 g) was extracted by maceration using 500 ml of ethanol-water (80:20). Antioxidant activity was estimated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) assays. In addition, Folin-Ciocalteu method was used to determine the total phenolic content. IC50 value of DPPH scavenging activity was 300 μg/mL. Total phenolic content was 25.3 mg as gallic acid equivalent/g extract. It seems that the phenolic constituents of the seeds are probably responsible for some part of antioxidant activity, while some unsaturated fatty acids (including linoleic and oleic acid) may be responsible for the other part,based on the review of the literature. Due to the limited studies about “Balangu” seed, more scientific surveys may be helpful for clarifying other biological properties of this traditionally important medicinal plant


Zargari A. Medicinal plants. 4th ed. Tehran: Tehran University of medical sciences publication; 1996. p.114.

Naghibi F, Mosaddegh M, Mohammadi- Motamed S,Ghorbani A. Labiatae Family in folk Medicine in Iran:from Ethnobotany to Pharmacology. Iranian Journal of PharmaceuticalResearch 2005; 2: 63-79

Aghili MH. Makhzan-al-advia. Edited by Rahimi R,Shams Ardakani MR, Farjadmand F. Tehran: Tehran University of medical sciences publication, 2009. p.207.

Nazem Jahan HMA. Exire-azam. Vol 3. Tehran:Research Institute for Islamic & Complementary Medicine 2008; p. 525.

Ghannadi A, Movahedian A, Jannesary Z.Hypocholesterolemic effects of Balangu (Lallemantia royleana) seeds in the rabbits fed on a cholesterolcontaining diet.Avicenna j of phytomed (AJP) 2014; 5:167-173.

Atabaki R, Hassanpour-ezatti M. Improvement of lidocaine local anesthetic action using Lallemantia royleana seed mucilage as an excipient. Iranian Journal ofPharmaceuticalResearch 2014; 13: 1431-36.?

[?7] Mahmood S, Hayat MQ, Sadiq A, Ishtiaq S, Malik S,AshrafM.Antibacterial activity ofLallemantia royleana (Benth.) indigenous to Pakistan. African Journal of MicrobiologyResearch 2013; 7: 4006-9.

Razavi SMA,MohammadiMoghaddamT,Mohammad AminiA. Physical-mechanical properties and chemical composition of Balangu (Lallemantia royleana ( Walla.)) seed. International Journal of Food Engineering 2008; 4: 1-10.

Khare CP. Indian medicinal plants. Berlin: Springer;2007. p. 360

Razavi SMA, Cui SW, Ding H. Structural and physicochemical characteristics of a novelwater-soluble gum from Lallemantia royleana seed. International Journal of Biological Macromolecules 2016; 83: 142-151.

Bahramparvar M, Hadad Khodaparast M, Razavi SMA. The effect of Lallemantia royleana (Balangu)seed, palmate-tuber salep and carboxymethylcellulose gums on the physicochemical and sensory properties of typical soft ice cream. International Journal of Dairy Technology 2009; 62: 571-76.

Sohail B, Huma N., MehmoodA,Abdullah M,Abbas Shah A. Use of tukhm-e-balangu (Lallemantia royleana) as a stabilizer in set type yogurt. Journal of Agroalimentary Processes and Technologies 2014; 20:247-56.

Vazirian M, Mohammadi M., Farzaei MH , Amin G ,Amanzadeh Y. Chemical composition and antioxidant activity ofOriganumvulgare subsp. vulgare essential oil from Iran. Research Journal of Pharmacognosy (RJP)2015; 2: 41-46.

Amanzadeh Y, Khosravi-Dehaghi. N., Gohari AR,Monsef-Esfehani HR, Sadat Ebrahimi SE. Antioxidant activity of essential oil of Lallemantia iberica in flowering stage and post- flowering stage. Research Journal of biological sciences 2011; 6: 114-117.

Mobli M, Haririan I, Amin G, Kamalinejad M,Hajimahmoodi M. Stability of polyphenols in Myrtle syrup, a traditional Iranian medicine. Traditional and integrativemedicine. 2016; 1: 35-39.

Amin G. Popular medicinal plants of Iran. Tehran:Iranian Research Institute of Medicinal Plants, Tehran:1991; p. 66

VeliogluYS,MazzaG,GaoL,OomahBD.Antioxidant Activity and Total Phenolics in Selected Fruits,Vegetables, and Grain Products. Journal of Agriculture andFoodChemistry. 1998;46: 4113-4117.

How to Cite
Bozorgi M, Vazirian M. Antioxidant Activity of Lallemantia royleana (Benth.) Seed Extract. Trad Integr Med. 1(4):147-150.
Research Article(s)