Chemical Composition, Total Phenolic Content, and Anti-Ulcerative Colitis Effects of Extract and Essential Oil of Cupressus arizonica Greene Fruits
-
Mohammad Bagher Majnooni
,
Saeid Mohammadi
,
Gholamreza Bahrami
,
Ehsan Mohammadi-Noori
,
Mohammad Hosein Farzaei
Abstract
Ulcerative colitis (UC) is a chronic inflammatory bowel disease, which has a global prevalence. Also, the plants of the Cupressaceae family have prominent anti-inflammatory and wound healing effects, so they can be considered as promising candidates for the treatment of UC. In this study, the therapeutic effects of extract and essential oil of Cupressus arizonica Greene (C. arizonica) fruits in the animal model of UC were investigated. Total of 35 Wistar male rats were treated with essential oil and hydroalcoholic extract for one week after induction of colitis by acetic acid. The colonic segment cut for macroscopic and histological analysis. The total amount of extract phenol and flavonoid content was assayed by Folin–Ciocalteu and aluminum chloride colorimetric method, respectively. The essential oil was analyzed by gas chromatography/mass spectrometer (GC/MS). The extract at doses of 100 mg/kg and 250 mg/kg and essential oil at doses of 0.5 mg/kg showed significant effects on UC (P < 0.05). The total phenolic content of hydroalcoholic extract in terms of mg of gallic acid/ g of extract was 191.625 ± 7.04 and the amount of total flavonoids in terms of mg of rutin/g of extract was 66.52 ± 6.51. Also, according to the results of GC/MS analysis, α-pinene was the major constituent of essential oil. Our results revealed that the extract and essential oil of C. arizonica fruits had therapeutic effects on UC, and this effect may be related to the presence of polyphenolic and terpene compounds.
Guan Q. A comprehensive review and update on the pathogenesis of inflammatory bowel disease. J Immunol Res 2019:7247238.
Ishihara S, Kawashima K, Fukuba N, Tada Y, Kotani S, et al. Irritable bowel syndrome-like symptoms in ulcerative colitis patients in clinical remission: association with residual colonic inflammation. Digestion 2019;99:46-51.
Berends SE, Strik AS, Löwenberg M, D’Haens GR, Mathôt RA. Clinical pharmacokinetic and pharmacodynamic considerations in the treatment of ulcerative colitis. Clin Pharmacokinet 2019;58(1):15-37.
Rubin DT, Ananthakrishnan AN, Siegel CA, Sauer BG, Long MD. ACG clinical guideline: ulcerative colitis in adults. American Journal of Gastroenterology. 2019;114(3):384-413.
Ko CW, Singh S, Feuerstein JD, Falck-Ytter C, Falck-Ytter Y, et al. AGA clinical practice guidelines on the management of mild-to-moderate ulcerative colitis. Gastroenterology 2019;156:748-764.
Levesque BG, Loftus EV. Medical management of ulcerative colitis: conventional therapy-azathioprine. Crohn's Disease and Ulcerative Colitis. Springer 2017; pp 413-419.
Nalagatla N, Falloon K, Tran G, Borren NZ, Avalos D, et al. Effect of accelerated infliximab induction on short-and long-term outcomes of acute severe ulcerative colitis: a retrospective multicenter study and meta-analysis. Clin Gastroenterol Hepatol 2019;17:502-509.
Tian C, Huang Y, Wu X, Xu C, Bu H, et al. The efficacy and safety of mesalamine and probiotics in mild-to-moderate ulcerative colitis: a systematic review and meta-analysis. Evid Based Complement Alternat Med 2020;2020:6923609.
Farzaei MH, Bahramsoltani R, Abdolghaffari AH, Sodagari HR, Esfahani SA, et al. A mechanistic review on plant-derived natural compounds as dietary supplements for prevention of inflammatory bowel disease. Expert Rev Gastroenterol Hepatol 2016;10:745-758.
Rahimi R, Nikfar S, Abdollahi M. Induction of clinical response and remission of inflammatory bowel disease by use of herbal medicines: a meta-analysis. World J Gastroenterol 2013;19:5738-5749.
Al-Mouhajer L, Chabo R, Saab AM, Saade K, Makhlouf H. Antibacterial activities of essential oils isolated from two species Cupressus arizonica Greene and Cupressus sempervirens L.(Var. horizontal and pyramidalis). Eur J Biomed Pharm Sci. 2017;4:430-435.
Khouaja W, Oliveira R, Raïes A, Dias ACP. Antifungal activity of the essential oils from Cupressus arizonica var. arizonica and var. glabra. Ind Crops Prod 2015;77:614-623.
Sedaghat MM, Dehkordi AS, Khanavi M, Abai MR, Mohtarami F, et al. Chemical composition and larvicidal activity of essential oil of Cupressus arizonica EL Greene against malaria vector Anopheles stephensi Liston (Diptera: Culicidae). Pharmacogn Res 2011;3:135-139.
Emami SA, Fakhrjafary M, Tafaghodi M, Hassanzadeh MK. Chemical composition and antioxidant activities of the essential oils of different parts of cupressus arizonica greene. J Essent Oil Res 2010;22:193-199.
Al‐Sayed E, Gad HA, El‐Shazly M, Abdel‐Daim MM, Nasser Singab A. Anti‐inflammatory and analgesic activities of cupressuflavone from Cupressus macrocarpa: Impact on pro‐inflammatory mediators. Drug Dev Res 2018;79:22-28.
Emami SA, Shahani A, Khayyat MH. Antioxidant activity of leaves and fruits of cultivated conifers in Iran. Jundishapur J Nat Pharm Prod 2013;8:113-117.
Al-Sayed E, Ke T-Y, Hwang T-L, Chen S-R, Korinek M, et al. Cytotoxic and anti-inflammatory effects of lignans and diterpenes from Cupressus macrocarpa. Bioorg Med Chem lett 2020;30:127127.
Lopéz L, Villavicencio M, Albores A, Martınez M, De la Garza J, et al. Cupressus lusitanica (Cupressaceae) leaf extract induces apoptosis in cancer cells. J Ethnopharmacol 2002;80:115-120.
Sadeghi-Aliabadi H, Emami SA, Saeidi M, Jafarian A. Cytotoxic effects of the extracts of Iranian Taxus baccata and Cupressus horizentalis on cancer cells. Iran J Pharm Res 2003;2:107-110.
Zhang J, Rahman AA, Jain S, Jacob MR, Khan SI, et al. Antimicrobial and antiparasitic abietane diterpenoids from Cupressus sempervirens. Res Rep Med Chem 2012;2:1-6.
Koriem KM, Gad IB, Nasiry ZK. Protective effect of Cupressus sempervirens extract against indomethacin-induced gastric ulcer in rats. Interdiscip Toxicol 2015;8:25-34.
Sepehrimanesh M, Samimi N, Koohi-Hosseinabadi O, Mokhtari M, Amiri-Zadeh, et al. Effects of Cupressus sempervirens extract on the healing of acetic acid-induced ulcerative colitis in rat. J Coloproctology 2018;38:309-313.
Chéraif I, Jannet HB, Hammami M, Khouja M, Mighri Z. Chemical composition and antimicrobial activity of essential oils of Cupressus arizonica Greene. Biochem Syst Ecology 2007;35:813-820.
Magalhães R, Torres D, Cavalcante R, Mota F, Moreira H, et al. Gastroprotective effect of alpha-pinene and its correlation with antiulcerogenic activity of essential oils obtained from Hyptis species. Pharmacogn Mag 2015;11:123-130.
Memariani Z, Sharifzadeh M, Bozorgi M, Hajimahmoodi M, Farzaei MH, et al. Protective effect of essential oil of Pistacia atlantica Desf. On peptic ulcer: role of α-pinene. J Tradit Chin Med 2017;37:57-63.
Minaiyan M, Karimi F, Ghannadi A. Anti-inflammatory effect of Pistacia atlantica subsp. kurdica volatile oil and gum on acetic acid-induced acute colitis in rat. Res J Pharmacogn 2015;2:1-12.
Al-Sayed E, Gad HA, El-Shazly M, Abdel-Daim MM, Nasser Singab A. Anti-inflammatory and analgesic activities of cupressuflavone from Cupressus macrocarpa: Impact on pro-inflammatory mediators. Drug Dev Res 2018;79:22-28.
Samimi N, Farjam M. The effect of cupressus sempervirens on ulcerative colitis: do pathological changes improve by oxidative stress amelioration? Galen Med J 2019;8:e1650.
Fakhri S, Jafarian S, Majnooni MB, Farzaei MH, Mohammadi-Noori E, et al. Anti-nociceptive and anti-inflammatory activities of the essential oil isolated from Cupressus arizonica Greene fruits. Korean J Pain 2022;35:33-42.
Harborne A. Phytochemical Methods a Guide to Modern Techniques of Plant Analysis: Springer Science & Business Media 1998.
Talebi M, Zarshenas MM, Yazdani E, Moein M. Preparation and evaluation of possible antioxidant activities of rose traditional tablet"(qurs-e-vard)" a selected traditional persian medicine (tpm) formulation via various procedures. Curr Drug Discov Technol 2021;18:e28092020186381.
Yazdani E, Talebi M, Zarshenas M, Moein M. Evaluation of possible antioxidant activities of barberry solid formulation, a selected formulation from Traditional Persian Medicine (TPM) via various procedures. Biointerface Res Appl Chem 2019;9:4517-4521.
Okayasu I, Hatakeyama S, Yamada M, Ohkusa T, Inagaki Y,et al. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology 1990;98:694-702.
Goyal N, Rana A, Ahlawat A, Bijjem KRV, Kumar P. Animal models of inflammatory bowel disease: a review. Inflammopharmacology 2014;22:219-233.
Somani SJ, Modi KP, Majumdar AS, Sadarani BN. Phytochemicals and their potential usefulness in inflammatory bowel disease. Phytother Res 2015;29:339-350.
Farzaei MH, Ghasemi-Niri SF, Abdolghafari AH, Baeeri M, Khanavi M, et al. Biochemical and histopathological evidence on the beneficial effects of Tragopogon graminifolius in TNBS-induced colitis. Pharm Biol 2015;53:429-436.
Patil MVK, Kandhare AD, Bhise SD. Effect of aqueous extract of Cucumis sativus Linn. fruit in ulcerative colitis in laboratory animals. Asian Pac J Trop Biomed 2012;2:S962-S969.
Kandhare AD, Raygude KS, Ghosh P, Ghule AE, Gosavi TP, et al. Effect of hydroalcoholic extract of Hibiscus rosa sinensis Linn. leaves in experimental colitis in rats. Asian Pac J Trop Biomed 2012;2:337-344.
El-Abhar HS, Hammad LN, Gawad HSA. Modulating effect of ginger extract on rats with ulcerative colitis. J Ethnopharmacol 2008;118:367-372.
Dundar E, Olgun EG, Isiksoy S, Kurkcuoglu M, Baser KHC, et al. The effects of intra-rectal and intra-peritoneal application of Origanum onites L. essential oil on 2, 4, 6-trinitrobenzenesulfonic acid-induced colitis in the rat. Exp Toxicol Pathol 2008;59:399-408.
Rezayat SM, Dehpour AR, Motamed SM, Yazdanparast M, Chamanara M, et al. Foeniculum vulgare essential oil ameliorates acetic acid-induced colitis in rats through the inhibition of NF-kB pathway. Inflammopharmacology 2018;26:851-859.
Rashidian A, Akbarzadeh D, Asgarpanah J, Dehpour A. Bunium persicum essential oil reduced acetic acid-induced rat colitis through suppression of NF-κB pathway. Avicenna J Phytomed 2021;11:505-514.
Jedidi S, Sammari H, Selmi H, Hosni K, Rtibi K, et al. Strong protective effects of Salvia officinalis L. leaves decoction extract against acetic acid-induced ulcerative colitis and metabolic disorders in rat. J Funct Foods 2021;79:104406.
El-Abhar HS, Hammad LNA, Gawad HSA. Modulating effect of ginger extract on rats with ulcerative colitis. J Ethnopharmacol 2008;118:367-372.
Rogler G, Andus T. Cytokines in Inflammatory bowel disease. World J Surg 1998;22:382-389.
Gautam MK, Goel S, Ghatule RR, Singh A, Nath G, et al. Curative effect of Terminalia chebula extract on acetic acid-induced experimental colitis: role of antioxidants, free radicals and acute inflammatory marker. Inflammopharmacology 2013;21:377-383.
Keshavarz A, Minaiyan M, Ghannadi A, Mahzouni P. Effects of Carum carvi L. (Caraway) extract and essential oil on TNBS-induced colitis in rats. Res Pharm Sci 2013;8:1-8.
Vezza T, Rodríguez-Nogales A, Algieri F, Utrilla MP, Rodriguez-Cabezas ME, et al. Flavonoids in inflammatory bowel disease: a review. Nutrients. 2016;8(4):211.
Kim D-S, Lee H-J, Jeon Y-D, Han Y-H, Kee J-Y, et al. Alpha-pinene exhibits anti-inflammatory activity through the suppression of MAPKs and the NF-κB pathway in mouse peritoneal macrophages. Am J Chin Med 2015;43:731-742.
Su Y-C, Hsu K-P, Hua K-F, Ho C-L. Composition, in vitro anti-inflammatory, antioxidant and antimicrobial activities of essential oils from leaf and twig parts of Cupressus cashmeriana. Nat Prod Commun 2015;10:1461-1464.
Tumen I, Süntar I, Keleş H, Küpeli Akkol E. A therapeutic approach for wound healing by using essential oils of Cupressus and Juniperus species growing in turkey. Evid Based Complement Alternat Med 2012;728281.
Farzaei MH, Rahimi R, Abdollahi M. The role of dietary polyphenols in the management of inflammatory bowel disease. Curr Pharm Biotech 2015;16:196-210.
Alabi QK, Akomolafe RO, Omole JG, Adefisayo MA, Ogundipe OL, et al. Polyphenol-rich extract of Ocimum gratissimum leaves ameliorates colitis via attenuating colonic mucosa injury and regulating pro-inflammatory cytokines production and oxidative stress. Biomed Pharmacother 2018;103:812-822.
Khodayar B, Farzaei MH, Abdolghaffari AH, Bahramsoltani R, Baeeri M, et al. The protective effect of the gallic acid against TNBS-induced ulcerative colitis in rats: role of inflammatory parameters. J Iran Med Council 2018;1:34-42.
Kim T, Song B, Cho KS, Lee I-S. Therapeutic potential of volatile terpenes and terpenoids from forests for inflammatory diseases. Int J Mol Sci 2020;21:2187.
Passos FRS, Araújo-Filho HG, Monteiro BS, Shanmugam S, Araújo AAdS, et al. Anti-inflammatory and modulatory effects of steroidal saponins and sapogenins on cytokines: A review of pre-clinical research. Phytomedicine 2022;96:153842.
Liu Y, Zhou M, Yang M, Jin C, Song Y, et al. Pulsatilla chinensis saponins ameliorate inflammation and dss-induced ulcerative colitis in rats by regulating the composition and diversity of intestinal flora. Front Cell Infect Microbiol 2021;11:728929.
Lu Q-G, Zeng L, Li X-H, Liu Y, Du X-F, et al. Protective effects of panax notoginseng saponin on dextran sulfate sodium-induced colitis in rats through phosphoinositide-3-kinase protein kinase B signaling pathway inhibition. World J Gastroenterol 2020;26:1156-1171.
Guo H, Wan B, Wang J, Zhang J, Yao W, et al. Astragalus saponins inhibit cell growth, aerobic glycolysis and attenuate the inflammatory response in a DSS-induced colitis model. Int J Mol Med 2019;43:1041-1048.
Afsharypuor S, Tavakoli P. Essential oil constituents of leaves and fruits of Cupressus arizonica Greene. J Essent Oil Res 2005;17:225-226.
Minaiyan M, Ghannadi A, Afsharipour M, Mahzouni P. Effects of extract and essential oil of Rosmarinus officinalis L. on TNBS-induced colitis in rats. Res Pharm Sci 2011;6:13-21.
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| Issue | Vol 7, No 3, 2022 | |
| Section | Research Article(s) | |
| DOI | https://doi.org/10.18502/tim.v7i3.10773 | |
| Keywords | ||
| Ulcerative colitis Phenolic compounds Flavonoids Terpenes Essential oil Hydroalcoholic extract α-Pinene | ||
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