Research Article

Formulation Development, Characterization and Antimicrobial Activity Evaluation of Sugar-Based and Sugar-Free Syrup Prepared with the Ingredients of Jushanda-Nazla


Jushanda (decoction), often used in traditional medicine, has many drawbacks like disagreeable taste, bulky dose, cumbersome preparation, and short stability. Therefore, in the present study, jushanda nazla (JN) was modified and optimized into a sugar-based (SBS) and sugar-free (SFS) syrup dosage form and evaluated for various physicochemical parameters, microbial contaminations and antimicrobial activities. JN was concentrated and reduced to minimum quantity by heating and twelve batches of SBS and five batches of SFS were prepared for optimization. Out of these, the best batch of SBS and SFS were selected based on consistency and minimum bulk. The best batches were further evaluated for the physicochemical parameter, microbial and heavy metal contamination. The finished products were also evaluated for antimicrobial activity against selected microbes. SBS was optimized with 170 mL JN reduced to 50 mL, sugar 40% (w/v) and consistency of one wire. SFS was optimized with 170 mL JN reduced to 30 mL, and 96 mg of aspartame. Organoleptic characters, ash value, viscosity, specific gravity and pH of finished products were satisfactory. The concentration of total phenolic, flavonoid, tannin, alkaloid and glycyrrhizin and chlorogenic acid were comparative in JN, SBS and SFS. SBS and SFS passed the WHO guideline for microbial and heavy metal contamination. Finished products showed significant antimicrobial activity against Streptococcus pyogenes, Streptococcus pneumoniae, Haemophilus influenzae and Salmonella. Analytical data of SBS and SFS showed significant optimistic results hence, generated data can be used for future reference. However, developed formulations should be further evaluated for their stability, safety and clinical efficacy.

Menditto E, Orlando V, De Rosa G, Minghetti P, Musazzi UM, et al. Patient centric pharmaceutical drug product design-the impact on medication adherence. Pharmaceutics 2020;12:44.

Yang Y, Ross J. Theories and concepts in the composition of Chinese herbal formulas. In: Chinese Herbal Formulas. Churchill Livingstone, Edinburgh, 2010.

Abraham JI, Mathew FL. Taste masking of paediatric formulation: A review on technologies, recent trends and regulatory aspects. Int J Pharm Pharm Sci 2014;6:12-19.

Goran MI, Ulijaszek SJ, Ventura EE. High fructose corn syrup and diabetes prevalence: A global perspective. Global Public Health 2013;8:55-64.

Winfield J, Rees J, Smith I. Pharmaceutical Practices. 4th Edition, Churchill Livingstone. Elsevier. Philadelphia 2009.

Fendrick AM, Monto AS, Nightengale B, Sarnes M. The economic burden of non-influenza-related viral respiratory tract infection in the United States. Arch Intern Med 2003; 163:487-494.

Eccles R. Understanding the symptoms of the common cold and influenza. Lancet Infect Dis 2005;5:718-725.

Fashner J, Ericson K, Werner S. Treatment of the common cold in children and adults. Am Fam Physician 2012;86:153-159.

Ghani N. Qarabadeen Najmul Ghani. Central Council for Research in Unani Medicine. New Delhi 2010.

Kabeeruddin M. Al Qarabadeen. Central Council for Research in Unani Medicine. New Delhi 2006.

Rowe RC, Sheskey PJ, Quinn ME. Handbook of pharmaceutical excipients. 6th Edition. Pharmaceutical Press. RPS Publishing. UK 2009.

Tandel KR. Sugar substitutes: Health controversy over perceived benefits. J Pharmacol Pharmacother 2011;2:236-243.

Pantone color chart. Available at URL: [Assessed on 05-07-2020].

Pawar HA, Joshi PR. Development and evaluation of taste-masked granular formulation of satranidazole by melt granulation technique. Journal of pharmaceutics 2014;789676.

The Unani Pharmacopoeia of India. part II. Volume II. 1st Edition. Department of AYUSH, Ministry of Health and Family Welfare, Government of India. New Delhi 2010.

Jyothiprabha V, Venkatachalam P. Preliminary phytochemical screening of different solvent extracts of selected Indian spices. Int J Curr Microbiol App Sci 2016;5:116-122.

Pandey A, Tripathi S. Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. J Pharmacogn Phytochem 2014;2:115-119.

Sanmugarajah V, Thabrew I, Sivapalan SR. Phyto, physicochemical standardization of medicinal plant Enicostemma littorale Blume. IOSR J Pharm 2013;3:52-58.

Miao S, Nie Q, Miao H, Zhang W, Mai K. Effects of dietary carbohydrate-to-lipid ratio on the growth performance and feed utilization of juvenile turbot (Scophthalmus maximus). J Ocean Univ China 2016;15:660-666.

Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 1959;31:426-428.

Jamuna SS, Karthika PK. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). J Appl Pharm Sci 2012;2:149-154.

Kavitha CCI, Indira G. Quantitative estimation of total phenolic, flavonoids, tannin and chlorophyll content of leaves of Strobilanthes kunthiana (Neelakurinji). J Med Plants 2016; 4:282-286.

John B, Sulaiman CT, George S, Reddy VR. Spectrophotometric estimation of total alkaloids in selected Justicia species. International journal of pharmacy and pharmaceutical sciences 2014;6:647-648.

Sethi PD. HPLC High-Performance Thin Layer Chromatography. CBS Publisher and Distributor Pvt. Ltd, New Delhi 2001.

Garbarino JR, Taylor HE. Inductively Coupled Plasma-Mass Spectrometric method for the determination of dissolved trace elements in natural water. U.S. Geological Survey, U.S Government 1996. Available at URL Accessed on 15-10-2017.

Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests (Chapter 61). USP 41, NF 36. Vol 4. US 2018.

Koeth LM, King A, Knight H, May J, Miller LA, et al. Comparison of cation-adjusted Mueller-Hinton broth with Iso-Sensitest broth for the NCCLS broth microdilution method. J Antimicrob Chemother 2000;46:369-376.

Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 2007;42:321-324.

Elshikh M, Ahmed S, Funston S, Dunlop P, McGaw M, et al. Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. Biotechnol Lett 2016;38:1015-1019.

Raqeequddin HM. Minhajus Saidla wal Kimiya. Idara Kitabul Shifa. New Dehli 2001.

Lohar DR. Protocol for Testing. Ayurvedic, Siddha, Unani Medicines Pharmacopoeial Laboratory for Indian Medicines. Depertment of AYUSH, Ministry of Health & Family Welfare, Government of India. Ghaziabad 2007.

Hossain MA. A phytopharmacological review on the Omani medicinal plant: Ziziphus jujube. J King Saud Univ Sci 2019;31:1352-1357.

Pastorino G, Cornara L, Soares S, Rodrigues F, Oliveira MB. Liquorice (Glycyrrhiza glabra): A phytochemical and pharmacological review. Phytother Res 2018;32:2323-2339.

Oza MJ, Kulkarni YA. Traditional uses, phytochemistry and pharmacology of the medicinal species of the genus Cordia (Boraginaceae). J Pharm Pharmacol 2017;69:755-789.

Ashraf MU, Muhammad G, Hussain MA, Bukhari SN. Cydonia oblonga M., a medicinal plant rich in phytonutrients for pharmaceuticals. Front Pharmacol 2016;7:163.

Gupta A, Maheshwari DK, Khandelwal G. Antibacterial activity of Glycyrrhiza glabra roots against certain gram-positive and gram-negative bacterial strains. J Applied Nat Sci 2013;5:459-464.

IssueVol 7, No 1, 2022 QRcode
SectionResearch Article(s)
Antimicrobial agent Chlorogenic acid Glycyrrhizin Jushanda Unani medicine

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How to Cite
Ali SZ, Rahman K, Sultana A. Formulation Development, Characterization and Antimicrobial Activity Evaluation of Sugar-Based and Sugar-Free Syrup Prepared with the Ingredients of Jushanda-Nazla. Trad Integr Med. 2022;7(1):87-103.