Research Article

Antipyretic, Analgesic and Anti-Inflammatory Activity of Qurs Afsanteen Saghir (A Polyherbal Unani Tablet) in Experimental Animals


Qurs Afsanteen Saghir is a polyherbal Unani formulation in the form of tablet. This formulation consists of multiple medicinal plants like Afsanteen (Artemisia absinthium L.), Badam Talkh (Prunus dulcis (Mill.) D.A.Webb), Asaroon (Asarum europaeum L.), Anisoon (Pimpinella anisum L.) and Tukhm-e-Karafs (Apium graveolens L.). The clinical adult dose of study drug is 3.5 –7 g per day as mentioned in Unani literature. The present study evaluated the antipyretic, analgesic and anti-inflammatory potential of Qurs Afsanteen Saghir using different animal models. Antipyretic activity was measured using yeast-induced pyrexia model in rats at 360 and 720 mg/kg bw dose of test drug and paracetamol (70 mg/kg bw p.o.) as standard control. Analgesic effect was evaluated using acetic acid-induced writhing test in mice using test drug at dose 720 and 1440 mg/kg bw and diclofenac sodium (15 mg/kg bw p.o.) as standard control. Eddy’s hot plate test was conducted in rats using test drug at the dose of 360 and 720 mg/kg bw and buprenorphine (0.10 mg/kg s.q.) as standard control. Anti-inflammatory activity was assessed by carrageenan-induced paw edema model in rats with the dose of 360 and 720 mg/kg of test drug and Indomethacin (10 mg/kg p.o.) as standard control. The study drug significantly reduced the temperature and pain at both dose levels in a time-dependent manner as compared to normal control. However, the reduction of inflammation was observed at low dose (360 mg/kg bw) only after 3 hours of carrageenan administration. These findings indicated that tested drug showed potential activity as antipyretic and analgesic; whereas the drug may not be considered quite effective as an anti-inflammatory agents.

Siddig A, Nooraini MA, Abdul BA, Elhassan MM, Ibrahim TAT. Energy dispersive X-ray microanalysis of elements and content of medicinal plants used traditionally as anticancer cure. Res J Biol Sci 2009;4:547-549.

Ong CKS, Lirk P, Tan CH, Seymour RA. An evidence-based update on non-steroidal anti-inflammatory drugs. Clin Med Res 2007;5:19-34.

Wijewickrama A, Abeyrathna G, Gunasena S, Idampitiya D. Effect of non-steroidal anti-inflammatory drugs (NSAIDS) on bleeding and liver in dengue infection, Oral Presentations: Emerging Infectious Diseases & One Health, 2016; 17th International Congress on Infectious Diseases/International Journal of Infectious Diseases 45S, 1-477.

Lanz R, Polster P, Brune K. Antipyretic analgesics inhibit prostaglandin release from astrocytes and macrophages similarly. Eur J Pharmacol 1986;130:105.

Pickering G, Loriot MA, Libert F, Eschalier A, Beaune P, et al. Analgesic effect of acetaminophen in humans: first evidence of a central serotonergic mechanism. Clin Pharmacol Ther 2006;79:371-378.

Ottani A, Leone S, Sandrini M, Ferrari A, Bertolini A. The analgesic activity of paracetamol is prevented by the blockade of cannabinoid CB1 receptors, Eur J Pharmacol 2006;531:280.

Parray SA, Bhat J, Iqbal SMF, Ahmad G, Jahan N, et al. Concept of obesity (Samane Mufrat) and its consequences in Greeko Arab medicine: A review. Int Pharm Sci 2012;2:1-8.

Jamal A, Parray SA, Siddiqui MA, Quamri MA. Concept of anxiety in greeco arab medicine: a review. Int Pharm Sci 2012;2:1-6.

Anjuma V, Arora P, Ansari SH, Najmi AK, Ahmad S. Antithrombocytopenic and immunomodulatory potential of metabolically characterized aqueous extract of Carica papaya leaves. Pharm Biol 2017;55:2043-2056.

Eccles R, Understanding the symptoms of the common cold and influenza, Lancet Infect Dis 2005;5:718-725.

Kabir al-Din HM. Alqarabadeen, 2nd ed. CCRUM. New Delhi 2006; p 937.

Arzani HMA. Qarabadeen-i-Qadri. CCRUM. New Delhi 2009; p 673.

Anonymous. The National Formulary Of Unani Medicine. CCRIUM Ministry of Health and Family Welfare. India 2006, Part-I; pp 8-9.

Reagan-Shaw S, Nihal M, Ahmad N. Dose translation from animal to human studies revisited. FASEB J 2008;22:659-6561.

Hajare SW, Chandra C, Tandan SK, Sarma J, Lal J, et al. Analgesic and antipyretic activities of dalbergia sissoo leaves, Indian J Pharmacol 2000;32: 357-360.

Abdulmalik IA, Sule MI, Musa AM, Yaro AH, Abdullahi MI, et al. Evaluation of analgesic and anti-Inflammatory effects of ethanol extract of ficus iteophylla leaves in rodents. Afr J Tradit Compleent Altern Med 2011;84:462-466.

Loux JJ, Depalma PD, Yankell SL, Antipyretic testing of aspirin in rats. Toxicol Appl Pharmacol 1972;22:672-675.

Liu X, Tang YP, Rui R, Jin Y, Guo JM, et al. Antipyretic and anti-inflammatory activities of Thais luteostoma extracts and underlying mechanisms. Chin J Nat Med. 2015;13:192-198.

Gupta AK, Parasar D, Sagar A, Choudhary V, Chopra BS, et al. Analgesic and anti-inflammatory properties of gelsolin in acetic acid induced writhing, tail immersion and carrageenan induced paw edema in mice. PLoS ONE 2015;10: e0135558.

Chouhan SY, Kataria CH, Goswami SC. Anti-Inflammatory activity of methanolic extract of brassica juncea seed on carrageenan induced paw edema in rats, Int J Pharm Sci Res 2014;5:3849-3851.

Young YH, Luo LY, Cheng YH, Hsieh CW, Liao CJ, et al. Analgesic and anti-inflammatory activities of [6]-gingerol. J Ethnopharmacol 2005;96:207-210.

Emim JA, Oleivera AB, Lapa AJ. Pharmacological evaluation of the anti-inflammatory activity of a citrus bioflavonoid, hesperidin, and the isoflavonoids, duartin and claussequinone, in Rats and Mice. J Pharm Pharmacol 1994,46: 118-122.

Gupta M, Mazumder UK, Kumar RS, Gomathi P, Rajeshwar Y, et al. Anti-inflammatory, analgesic and antipyretic effects of methanol extract from bauhinia racemosa stem bark in animal models. J Ethnopharmacol 2005;98:267-273.

Zafar MM, Hamdard ME, Hameed A. Screening of artemisia absinthium for ant malarial effects on plasmodium berghei in mice: a preliminary report Department of Microbiology, Faculty of Science, Jam z Hamdard (Hamdard University), Indian J Ethnopharmacol 1990;30:223-226.

Yu-Chin L, Ching-Wen C, Chi-Rei W. Antitussive, anti-pyretic and toxicological evaluation of Ma-Xing-Gan-Shi-Tang in rodents. BMC Complement Altern Med 2016;16:456.

Shojaii A, Motaghinejad M, Norouzi SM. Evaluation of anti-inflammatory and analgesic activity of the extract and fractions of astragalus hamosus in animal models. Iran J Pharm Res 2015;14:263-269.

Tas A, Hanefi O, Nazmi A, Enes Altug M. Evaluation of analgesic and anti-inflammatory activity of Pimpinella anisum fixed oil extract. Indian vet J 2006;83:840-843. ·

Atta AH, Alkofahi A. Anti-nociceptive and anti-inflammatory effects of some Jordanian medicinal plant extracts. J Ethnopharmacol 1998;60:117-124.

Hadi A, Nazari H, Parvini S, Nazari N, Mohammadi A. Anti-inflammatory and analgesic activities of artemisia absinthium and chemical composition of its essential oil. Int J Pharm Sci Rev 2014;24:237-244.

Ghlissi Z, Kallel R, Krichen F, Hakim A, Zeghal K, et al. Polysaccharide from pimpinella anisum seeds: structural characterization, anti-inflammatory and laser burn wound healing in mice. Int J Biol Macromol 2020;156:1530-1538.

IssueVol 6, No 4, 2021 QRcode
SectionResearch Article(s)
Polyherbal Unani Apium graveolens Artemisia absinthium Asarum europaeum

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Maseehullah M, Husain GM, Zakir M, Husain MK, Javed G, Kazmi MH, Urooj M. Antipyretic, Analgesic and Anti-Inflammatory Activity of Qurs Afsanteen Saghir (A Polyherbal Unani Tablet) in Experimental Animals. Trad Integr Med. 2021;6(4):320-332.