Research Article

Chemical Composition, Radical Scavenging and β-carotene Bleaching Assay of Essential Oils from Citrus aurantifolia, Citrus sinensis Peel, and Zataria multiflora Aerial Parts

Abstract

Essential oil obtained from medicinal plants has been shown to have different biological activities and could replace chemical antioxidants to decrease oxidation of toxic chemical constituents and prevent disorders associated with oxidative damages. This study was aimed to evaluate chemical compositions and antioxidant activities of the essential oils obtained from Citrus aurantifolia (lime), Citrus sinensis (orange) peel, and Zataria multiflora aerial parts growing in Iran. The chemical composition and antioxidant activities of essential oils were examined using 2, 2-diphenyl-1- picrylhydrazyl (DPPH) scavenging activity and β-carotene bleaching methods. The results were compared with butylated hydroxyl toluene as a synthetic antioxidant. The chemical compositions of essential oils were analyzed with gas chromatography and mass spectrometry. Limonene (40.33%), β-pinene (9.45%), α-terpineol (10.88%), and γ-terpinolene (8.89%) were identified as the major compounds of the oil from C. aurantifolia peel. The main component in the oil of C. sinensis peel was  limonene (90.492%), and thymol (38.67%), carvacrol  (15.29%), p-cymene (10.23%), and γ-terpinene (9.75%) were the main components in the essential oil obtained from Z. multiflora. Z. multiflora essential oil showed potent antioxidant activity by DPPH (76%) and β-carotene bleaching (73.3%) methods. This study indicated that Z. multiflora essential oil exhibited the highest radical scavenging effect and could be used as an obtainable source of natural antioxidant.

Blokhina O, Virolainen E, Fagerstedt KV.Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany 2003; 19: 179-94.

Halliwell B. Antioxidant defence mechanisms: from the beginning to the end (of the beginning). Free Radic Res 1999; 31(4): 261-72.

Abdollahi M, Ranjbar A, Shadnia S, Nikfar S, Rezaie A. Pesticides and oxidative stress: a review. Med Sci Monit 2004; 10(6): RA141-RA147.

Chauhan V, Chauhan A. Oxidative stress in Alzheimer's disease. Pathophysiology 2006; 13(3):195-208.

Al-Qarawi AA, Abdel-Rahman H, Ali BH, Mousa HM, El-Mougy SA. The ameliorative effect of dates (Phoenix dactylifera L.) on ethanol-induced gastric ulcer in rats. J Ethnopharmacol 2005; 98(3):313-7.

Velioglu YS, Mazza G, Gao L, Oomah BD.Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 1998; 46(10): 4113-7.

Frankel EN. Review. Recent advances in lipid oxidation. Journal of the Science of Food and Agriculture 1991; 54(4): 495-511.

Song HS, Ukeda H, Sawamura M. Antioxidative activities of citrus peel essential oils and their components against linoleic acid oxidation. Food Science and Technology Research 2001; 7(1): 50-6.

Yanishlieva NV, Marinova EM, Gordon MH, Raneva VG. Antioxidant activity and mechanism of action of thymol and carvacrol in two lipid systems. Food Chemistry 1999; 64(1): 59-66.

Che Man Y, Jaswir I. Effect of rosemary and sage extracts on frying performance of refined, bleached and deodorized (RBD) palm olein during deep-fat frying. Food Chemistry 2000; 69(3): 301-7.

Patil JR, Jayaprakasha GK, Chidambara Murthy KN, Tichy SE, Chetti MB, Patil BS. Apoptosis- mediated proliferation inhibition of human colon cancer cells by volatile principles of Citrus aurantifolia. Food Chemistry 2009; 114(4): 1351-8.

Ghasemi K, Ghasemi Y, Ebrahimzadeh MA.Antioxidant activity, phenol and flavonoid contents of 13 citrus species peels and tissues. Pak J Pharm Sci 2009; 22(3): 277-81.

Ahmad A, Khan A, Akhtar F, Yousuf S, Xess I, Khan LA, et al. Fungicidal activity of thymol and carvacrol by disrupting ergosterol biosynthesis and membrane integrity against Candida. Eur J Clin Microbiol Infect Dis 2011; 30(1): 41-50.

Mahammadi Purfard A, Kavoosi GH. Chemical composition, radical scavenging, antibacterial and antifungal activities of zataria multiflora bioss essential oil and aqueous extract. Journal of Food Safety 2012; 32(3): 326-32.

Adams RP. Identification of essential oilcomponents by gas chromatography/mass spectorscopy. Carol Stream, IL: Allured Publishing Corporation; 2007. p. 456.

Pokorny J, Yanishlieva N, Gordon MH.Antioxidants in food: practical applications. BocaRaton, FL: CRC Press; 2001.

Moure A, Franco D, Sineiro J, Dominguez H, Nunez MJ, Lema JM. Evaluation of extracts from Gevuina avellana hulls as antioxidants. J Agric Food Chem 2000; 48(9): 3890-7.

Stationery Office. British pharmacopoeia (veterinary). London: Stationery Office; 2008.

Sweetman SC. Martindale: The complete drug reference. 36th ed. Gurnee, IL: Pharmaceutical Press; 2009.

Sadeghzadeh L, Sefidkon F, Olya P. Chemical composition and antimicrobial activity of essential oil of Zataria multiflora. Pajouhesh Va Sazandegi 2006; 19(1): 52-65. [In Persian].

Karimian P, Kavoosi G, Saharkhiz MJ.Antioxidant, nitric oxide scavenging and malondialdehyde scavenging activities of essential oil from different chemotypes of z. multiflora. Natural Product Research 2012; 26(22): 2144-7.

Zomorodian K, Saharkhiz MJ, Rahimi MJ,Bandegi A, Shekarkhar G, Bandegani A, et al. Chemical composition and antimicrobial activities of the essential oils from three ecotypes of Zataria multiflora. Pharmacogn Mag 2011; 7(25): 53-9.

Bamdad F, Kadivar M, Keramat J. Evaluation of phenolic content and antioxidant activity of Iranian caraway in comparison with clove and BHT using model systems and vegetable oil. International Journal of Food Science & Technology 2006;41(Suppl 1): 20-7.

Tepe B, Sihoglu-Tepe A, Daferera D, Polissiou M, Sokmen A. Chemical composition and antioxidant activity of the essential oil of Clinopodium vulgare L. Food Chemistry 2007;103(3): 766-70.

Duenas M, Hernandez T, Estrella I. Assessment of in vitro antioxidant capacity of the seed coat and the cotyledon of legumes in relation to their phenolic contents. Food Chemistry 2006; 98(1): 95-103.

Katalinic V, Milos M, Kulisic T, Jukic M.Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry 2006; 94(4): 550-7.

Theriault M, Caillet S, Kermasha S, Lacroix M.Antioxidant, antiradical and antimutagenic activities of phenolic compounds present in maple products. Food Chemistry 2006; 98(3): 490-501.

Proteggente AR, Saija A, de Pasquale A, Rice-Evans CA. The compositional characterisation and antioxidant activity of fresh juices from sicilian sweet orange (Citrus sinensis L. Osbeck) varieties. Free Radic Res 2003; 37(6): 681-7.

Files
IssueVol 1, No 2, 2016 QRcode
SectionResearch Article(s)
Keywords
Citrus aurantifolia Citrus sinensis Zataria multiflora 2-Diphenyl-1-picrylhydrazyl; β-Carotene Antioxidant activity

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
Ghazian F, Sadati SN, Khanavi M, Mohammad-Taghizadeh Kashani L. Chemical Composition, Radical Scavenging and β-carotene Bleaching Assay of Essential Oils from Citrus aurantifolia, Citrus sinensis Peel, and Zataria multiflora Aerial Parts. Trad Integr Med. 2016;1(2):59-65.