Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds
Abstract
Invertase, the key enzyme responsible for sucrose hydrolysis. Inhibition of invertase can decrease the postprandial blood sugar level in diabetic patients and keep the blood glucose level normal where cytotoxicity to fast-growing cells like those of brine shrimp (Artemia salina) nauplii is a great measurement for further important drug development like anticancer drugs. This study was aimed to investigate potential anti-diabetic and cytotoxic activities of the ethanol and acetone extracts of Swietenia macrophylla leaves, Syzygium cumini and Trigonella foenum-graecum seeds. Invertase inhibition activities of S. macrophylla leaves, S. cumini, and T. foenum-graecum seeds were measured by spectrophotometrically using standard protocols and cytotoxicities were measured by brine shrimp lethality bioassay. Among the plant extracts, all ethanol extracts showed higher invertase inhibition activities than all acetone extracts. S. cumini seed ethanol extract showed the highest invertase inhibition activity whereas S. macrophylla leaves acetone extract showed the lowest invertase inhibition activity. The maximum toxicity was observed in ethanol extract of T. foenum-graecum seed whereas the lowest toxicity was observed in acetone extract of S. macrophylla leaves. Both ethanol and acetone extract of T. foenum-graecum seeds showed significant cytotoxic activities. This investigation suggested that S. cumini and T. foenum-graecum seeds possess potential antidiabetic activities and T. foenum-graecum seeds have potential cytotoxicity.
Belle TLV, Coppieters KT, Herrath MGV. Type 1 diabetes: etiology, immunology, and therapeutic strategies. Physiol Rev 2011;91:79-118.
Poovitha S, Parani M. In vitro and in vivo α-amylase and α-glucosidase inhibiting activities of the protein extracts from two varieties of bitter gourd (Momordicacharantia L.). BMC Complement Altern Med 2016;16:185.
van de Laar FA. Alpha-glucosidase inhibitors in the early treatment of type 2 diabetes. Vasc Health Risk Manag 2008;4:1189.
Etxeberria U, de la Garza AL, Campión J, Martinez JA, Milagro FI. Antidiabetic effects of natural plant extracts via inhibition of carbohydrate hydrolysis enzymes with emphasis on pancreatic alpha amylase. Expert Opin Ther Tar 2012;16:269-297.
Preethi R, Devanathan V, Loganathan P. Antimicrobial and antioxidant efficacy of some medicinal plants against food borne pathogens. Adv Biol Res 2010;4:122-125.
Guariguata L, Whiting DR, Hambleton I, Beagley J, Linnenkamp U, Shaw JE. Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes Res Clin Pract 2014;103:137-149.
Anderson JE, Goetz CM, McLaughlin JL, Suffness M. A blind comparison of simple bench‐top bioassays and human tumour cell cytotoxicities as antitumor prescreens. Phytochem Analysis 1991;2:107-111.
D’Britto V, Devi PP, Prasad BLD, Dhawan A, Mantri VG, Prabhune A. Medicinal plant extracts used for blood sugar and obesity therapy shows excellent inhibition of invertase activity: synthesis of nanoparticles using this extract and its cytotoxic and genotoxic effects. Int J life Sci Pharma Res 2012;2:61-74.
Fatmawati S, Shimizu K, Kondo R. Ganoderol B: a potent α-glucosidase inhibitor isolated from the fruiting body of Ganodermalucidum. Phytomedicine 2011;18:1053-1055.
Kawamura-Konishi Y, Watanabe N, Saito M, Nakajima N, Sakaki T, Katayama T. Isolation of a new phlorotannin, a potent inhibitor of carbohydrate-hydrolyzing enzymes, from the brown alga Sargassum patens. J Agric Food Chem 2012;60:5565-5570.
Orhan N, Aslan M, Şüküroğlu M, Orhan DD. In vivo and in vitro antidiabetic effect of Cistus laurifolius L. and detection of major phenolic compounds by UPLC–TOF-MS analysis. J Ethnopharmacol 2013;146:859-865.
Panwar H, Calderwood D, Grant IR, Grover S, Green BD. Lactobacillus strains isolated from infant feces possess potent inhibitory activity against intestinal alpha-and beta-glucosidases suggesting anti-diabetic potential. Eur J Nutr 2014;53:1465-1474.
Ali RB, Atangwho IJ, Kuar N, Ahmad M, Mahmud R, Asmawi MZ. In vitro and in vivo effects of standardized extract and fractions of Phaleriamacrocarpa fruits pericarp on lead carbohydrate digesting enzymes. BMC Complement Altern Med 2013;13:39.
Kim KT, Rioux LE, Turgeon SL. Alpha-amylase and alpha-glucosidase inhibition is differentially modulated by fucoidan obtained from Fucusvesiculosus and Ascophyllumnodosum. Phytochemistry 2014;98:27-33.
Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med 1982;45:31-34.
Michael AS, Thompson CG, Abramovitz M. Artemiasalina as a test organism for bioassay. Science 1956;123:464-464.
Vanhaecke P, Persoone G, Claus C, Sorgeloos P. Proposal for a short-term toxicity test with Artemianauplii. Ecotox Environ Safe 1981;5:382-387.
Sleet RB, Brendel K. Improved methods for harvesting and counting synchronous populations of Artemianauplii for use in developmental toxicology. Ecotox Environ Safe 1983;7:435-446.
Riss TL, Moravec R. Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays. Assay Drug Dev Techn 2004;2:51-62.
Martınez M, Ramo JD, Torreblanca A, Dıaz-Mayans J. Effect of cadmium exposure on zinc levels in the brine shrimp Artemiaparthenogenetica. Aquaculture 1999;172:315-325.
Barahona MV, Sanchez-Fortun S. Toxicity of carbamates to the brine shrimp Artemiasalina and the effect of atropine, BW284c51, iso-OMPA and 2-PAM on carbaryl toxicity. Environ Pollut 1999;104:469-476.
Pelka M, Danzl C, Distler W, Petschelt A. A new screening test for toxicity testing of dental materials. Int J Dent 2000;28:341-345.
Dewanjee S, Maiti A, Das AK, Mandal SC, Dey SP. Swietenine: A potential oral hypoglycemic from Swieteniamacrophylla seed. Fitoterapia 2009;80:249-251.
Kumar A, Ilavarasan R, Jayachandran T, Deecaraman M, Aravindan P, Padmanabhan N. Anti-diabetic activity of Syzygiumcumini and its isolated compound against streptozotocin-induced diabetic rats. J Med Plants Res 2008;2:246-249.
Vats V, Grover JK, Rathi SS. Evaluation of anti-hyperglycemic and hypoglycemic effect of Trigonellafoenum-graecum Linn, Ocimum sanctum Linn and Pterocarpus marsupium Linn in normal and alloxanized diabetic rats. J Ethnopharmacol 2002;79:95-100.
Goh BH, Kadir HA. In vitro cytotoxic potential of Swieteniamacrophylla King seeds against human carcinoma cell lines. J Med Plants Res 2011;5:1395-1404.
Afify A, Fayed SA, Shalaby EA, El-Shemy HA. Syzygiumcumini (pomposia) active principles exhibit potent anticancer and antioxidant activities. Afr J Pharm Pharmacol 2011;5:948-956.
Nazif NM. The anthocyanin components and cytotoxic activity of Syzygiumcumini (L.) fruits growing in Egypt. Nat Prod Sci 2007;13:135-139.
Ali MS, Amin MR, Kamal CMI, Hossain MA. In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves. Asian Pac J Trop Biomed 2013;3:464-469.
Sathishkumar T, Anitha S, Sharon RE, Santhi V, Sukanya M, Kumaraesan K. Evaluation of in vitro invertase inhibitory activity of Manilkarazapota seeds–a novel strategy to manage diabetes mellitus. J Food Biochem 2015;39:517-527.
Melius P. Isolation of yeast invertase by sephadex gel chromatography. A biochemistry laboratory experiment. J Chem Educ 1971;48:765.
Hossain S, Kader G, Nikkon F, Yeasmin T. Cytotoxicity of the rhizome of medicinal plants. Asian Pac J Trop Biomed 2012;2:125-127.
Asano N. Glycosidase inhibitors: update and perspectives on practical use. Glycobiology 2003;13:93-104.
Gray AM, Flatt PR. Nature’s own pharmacy: The diabetes perspective. Proc Nutr Soc 1997;56:507-517.
Swanston-Flatt SK, Flatt PR, Day C, Bailey CJ. Traditional dietary adjuncts for the treatment of Diabetes mellitus. Proc Nutr Soc 1991;50:641-650.
Rajan M, Kumar VK, Kumar PS, Swathi KR, Haritha S. Antidiabetic, antihyperlipidaemic and hepatoprotective activity of methanolic extract of Ruellia tuberose Linn. leaves in normal and alloxan induced diabetic rats. J Chem Pharm Res 2012;4:2860- 2868.
Deciga-Campos M, Rivero-Cruz I, Arriaga-Alba M, Castaneda-Corral G, Angeles-Lopez GE, Navarrete A. Acute toxicity and mutagenic activity of Mexican plants used in traditional medicine. J Ethnopharmacol 2007;110:334-342.
| Files | ||
| Issue | Vol 5, No 2, 2020 | |
| Section | Research Article(s) | |
| DOI | https://doi.org/10.18502/tim.v5i2.3626 | |
| Keywords | ||
| Plant extracts Antidiabetic activity Invertase inhibition activit Cytotoxicity | ||
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