Evaluation of the Effects of Nano-honey Scaffold on Reconstruction of Calvarial Defects in Rat
AbstractBone defects have caused many problems. Restoration of bone defects with great gaps, has been a huge problem for many patients. Nowadays, different ways are used for bone restorations that cause difficulties; so there is tendency to use organic drugs such as propolis. The aim of this study was investigating the effects of nono-honey on calvarial defect. In this research 20 Wistar male rats were divided into two control and nano-honey groups. After general anesthesia, to access the calvaria, the midline was cut. We turned the skin and periosteum over and dig a fossa in parietal bone with electronic handpiece with 1000 round per minute (depth 5 mm and length 6 mm). After 8 weeks, histopathologic samples were provided and the new vessels, neutrophils, lymphocytes, macrophage, fibroblast, granulation tissue and formation of fresh bone trabeculae were measured. We observed that average number of neutrophils in nano-honey group was lower than control group, expressively (p = 0.030). The average of bone trabuculation in nano-honey group was more than other group (p < 0.001). Other histological factors was not significantly different (p > 0.05). In this study, it was shown nano-honey has positive effects on restoration of hard and soft tissues. Since this research was carried out on rats more study and examinations on human beings are needed. Furthermore, adding osteoconductive and stimulant of regenerating bones in order to evaluate the effects on restoration is recommended.
Cavalcanti SC, Pereira CL, Mazzonetto R, De Moraes M, Moreira R. Histological and histomorphometric analyses of calcium phosphate cement in rabbit calvaria. J Craniomaxillofac Surg 2008;36:354-359.
Schroeder JE, Mosheiff R. Tissue engineering approaches for bone repair: concepts and evidence. Injury 2011;42:609-613.
Bayat M, Heravi FM, Mahmoudi M, Bahrami N. Bone reconstruction following application of bone matrix gelatin to alveolar defects: a randomized clinical trial. Int J Organ Transplant Med 2015;6:176.
Hoffer MJ, Griffon DJ, Schaeffer DJ, Johnson AL, Thomas MW. Clinical Applications of Demineralized Bone Matrix: A Retrospective and Case‐Matched Study of Seventy Five Dogs. Vet Surg 2008;37:639-647.
Bahrami N, Bayat M, Mohamadnia A, Khakbiz M, Yazdankhah M. Purmorphamine as a Shh signaling activator small molecule promotes motor neuron differentiation of mesenchymal stem cells cultured on nanofibrous PCL scaffold. Mol Neurobiol 2017;54:5668-5675.
Bahrami N, Malekolkottab F, Ebrahimi-Barough S, Alizadeh Tabari Z, Hamisi J, Kamyab A. The effect of purmorphamine on differentiation of endometrial stem cells into osteoblast-like cells on collagen/hydroxyapatite scaffolds. Artif Cells Nanomed Biotechnol 2017;45:1343-1349.
Bayat M, Shojaei S, Bahrami N, Mohamadnia AR, Shojaei P, Bahrami N. Protein engineering of recombinant Human Bone Morphogenetic Protein 2 with higher interaction with Ca phosphate based scaffold usedfor osteogenesis. J Biomed Mater Res A. 2017.
Schwartz Z, Doukarsky‐Marx T, Nasatzky E, Goultschin J, Ranly D, Greenspan D. Differential effects of bone graft substitutes on regeneration of bone marrow. Clin Oral Implants Res. 2008;19:1233-1345.
Harborne JB, Williams CA. Advances in flavonoid research since 1992. Phytochem 2000;55:481-504.
Chirumbolo S. Flavonoids in propolis acting on mast cell-mediated wound healing. Inflammopharmacology 2012;20:99-101.
Momen-Beitollahi J, Mansorian A, Esmaili M, Amanlou M, Mohamadnia A, Bahrami N. Antimicrobial effects of propolis extract on the most prevalent oral pathogens: an in vitro study. J Islam Dent Assoc Iran 2009;21.
Seidel V, Peyfoon E, Watson DG, Fearnley J. Comparative study of the antibacterial activity of propolis from different geographical and climatic zones. Phytotherapy Res 2008;22:1256-1263.
Orsi R, Sforcin J, Rall V, Funari S, Barbosa L, Fernandes J. Susceptibility profile of Salmonella against the antibacterial activity of propolis produced in two regions of Brazil. J Venom Anim Toxins Incl Trop Dis 2005;11:109-116.
Molan PC. The antibacterial activity of honey: 1. The nature of the antibacterial activity. Bee world. 1992;73:5-28.
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-582.
Magro O, De carvalho A. Application of propolis to dental sockets and skin wounds. J Nihon Univ Sch Dent. 1990;32:4-13.
Ai J, Heidari-Keshel S, Azami M, Ai A, Bahrami N, Mohamadnia A. Repair of critical size rat calvarial defects using endometrial-derived stem cells embedded within gelatin/apatite nanocomposite scaffold. Stem Cell Discovery 2013;3:37-43.
Park YK, Alencar SM, Aguiar CL. Botanical origin and chemical composition of Brazilian propolis. J Agric Food Chem 2002;50:2502-2506.
Scheller S, Krol W, Owczarek S, Swiacik J, Gabrys J, Shani J. Antitumoral property of ethanolic extract of propolis in mice-bearing Ehrlich carcinoma, as compared to bleomycin. Z Naturforsch C Bio Sci 1989;44:1063-1065.
Velazquez C, Navarro M, Acosta A, Angulo A, Dominguez Z, Robles R. Antibacterial and free radical scavenging activities of Sonoran propolis. J Appl Microbiol. 2007;103:1747-1756.
Schmidt BL, Mahan D, Kearns G. The removal of plates and screws after Le Fort I osteotomy. J Oral Maxillofac Surg 1998;56:184-188.
Agins H, Alcock N, Bansal M, Salvati E, Wilson P, Pellicci P, et al. Metallic wear in failed titanium-alloy total hip replacements. J Bone Joint Surg A 1988;70:347-356.
Katou F, Andoh N, Motegi K, Nagura H. Immuno-inflammatory responses in the tissue adjacent to titanium miniplates used in the treatment of mandibular fractures. J Craniomaxillofac Surg 1996;24:155-162.
Rosenberg A, Grätz K, Sailer H. Should titanium miniplates be removed after bone healing is complete? Int J Oral Maxillofac Surg 1993;22:185-188.
Jorgenson DS, Mayer MH, Ellenbogen RG, Centeno JA, Johnson FB, Mullick FG. Detection of titanium in human tissues after craniofacial surgery. Plast Reconstr Surg 1997;99:976-979.
Kim Y-K, Yeo H-H, Lim S-C. Tissue response to titanium plates: a transmitted electron microscopic study. J Oral Maxillofac Surg 1997;55:322-326.
Schliephake H, Reiss G, Urban R, Neukam F, Guckel S. Metal release from titanium fixtures during placement in the mandible: an experimental study. Int J Oral Maxillofac Implants 1993;8:23-25.
Hunt J, Williams D, Ungersböck A, Perrin S. The effect of titanium debris on soft tissue response. J Mater Sci Mater Med 1994;5(6):381-383.
Lalor P, Revell P, Gray A, Wright S, Railton G, Freeman M. Sensitivity to titanium. A cause of implant failure? Bone Joint J 1991;73:25-28.
Castillo MH, Button TM, Doerr R, Homs MI, Pruett CW, Pearce JI. Effects of radiotherapy on mandibular reconstruction plates. Am J Surg 1988;156:261-263.
Sullivan PK, Smith JF, Rozzelle AA. Cranio-orbital reconstruction: safety and image quality of metallic implants on CT and MRI scanning. Plast Reconstr Surg. 1994;94:589-596.
Brodke DS, Gollogly S, Mohr RA, Nguyen B-K, Dailey AT, Bachus KN. Dynamic cervical plates: biomechanical evaluation of load sharing and stiffness. Spine 2001;26:1324-1329.
Keshel SH, Soleimani M, Tavirani MR, Ebrahimi M, Raeisossadati R, Yasaei H. Evaluation of unrestricted somatic stem cells as a feeder layer to support undifferentiated embryonic stem cells. Mol Reprod Dev 2012;79:709-718.
Hemalatha R, Hemagaran G. Effectiveness of Honey and Aloe Vera on Post Extraction Healing. IOSR-J Dent Med Science 2015;14:6.
Okeniyi JA, Olubanjo O, Ogunlesi TA, Oyelami OA. Comparison of healing of incised abscess wounds with honey and EUSOL dressing. J Altern Complement Med 2005;11:511-513.