Tehran University of Medical Sciences Traditional and Integrative Medicine 2476-5104 7 1 2022 03 09 52 63 Ardeshir Moayeri Department of Anatomy, Medical School, Ilam University of Medical Sciences, Ilam, Iran AND Biotechnology and Medicinal Plants Research Center, Medical School, Ilam University of Medical Sciences, Ilam, Iran Kholoud Ramz Department of Anatomy, Medical School, Ilam University of Medical Sciences, Ilam, Iran Elahe Karimi Biotechnology and Medicinal Plants Research Center, Medical School, Ilam University of Medical Sciences, Ilam, Iran Monireh Azizi Department of Anatomy, Medical School, Ilam University of Medical Sciences, Ilam, Iran Naser Abbasi Biotechnology and Medicinal Plants Research Center, Medical School, Ilam University of Medical Sciences, Ilam, Iran AND Department of Pharmacology, Medical School, Ilam University of Medical Sciences, Ilam, Iran Ali Aidy Biotechnology and Medicinal Plants Research Center, Medical School, Ilam University of Medical Sciences, Ilam, Iran Mahmoud Bahmani Biotechnology and Medicinal Plants Research Center, Medical School, Ilam University of Medical Sciences, Ilam, Iran 2021 06 01 2021 09 19 Finding more efficient agents with fewer side effects for the treatment of burns has been a concern for researchers. The current study aims to assess Aloe vera (L.) Burm.f. and Vitis vinifera L. extract combination (AVEC) effects on wound healing in rats compared with silver sulfadiazine (SSD). Identification of individual polyphenols of the plant extracts was performed by HPLC. The animals were randomly divided into twelve groups. Standard second-degree burn wounds were induced on the back. Groups were treated with Aloe vera (A.V) and Vitis vinifera (V.V) creams at concentrations of 0.5, 1, 1.5, and 2% with ED50 values of 1.5 ± 0.02 and 1.4 ± 0.08 %, respectively. The other group was treated with an AVEC cream (1.5%). The samples of burned skin tissue were collected from the rats for histopathological examination. To evaluate the expression of VEGF and TGFβ1, the real time-PCR method was used. Kaempferol was only detected in Aloe. This study revealed that AVEC cream exhibits significant wound healing activity. VEGF and TGFβ1 had a significant increase in the AVEC. Based on our findings, AVEC cream can be a therapy of choice for burn injuries. https://jtim.tums.ac.ir/index.php/jtim/article/view/443