Tehran University of Medical Sciences Traditional and Integrative Medicine 2476-5104 1 3 2016 10 04 115 121 EN Mohammad-Khalil Kargar-Shoragi Department of Sport Physiology, School of Physical Education, University of Sistan and Baluchestan, Zahedan, Iran Mohsen Ghofrani Department of Sport Physiology, School of Physical Education, University of Sistan and Baluchestan, Zahedan, Iran Laleh Bagheri Department of Physical Education, School of Literature and Humanities, Shahrekord University, Shahrekord, Iran Saeid Emamdoost Tehran University of Medical Sciences, Tehran, Iran Khadijeh Otadi Department of Physical Therapy, School of Rehabilitation, Tehran University of Medical Sciences, Tehran, Iran 2016 10 02 2016 10 02 Physical exercise acts as a mechanical stressor that can provoke biochemical alterations. Accordingly, muscular effort is able to modify blood concentrations in some cellular enzymes. Cupping is one of the traditional methods used to restore and revive the balance of the budy. This study aimed to evaluate the impact of cupping and a practice session on the level of some liver enzymes in men handball team members of Zahedan city, Iran. The participants were all the members of the Zahedan men handball team with the mean age of 23.66 ± 5.86 years, mean height of 177.19 ± 4.58 cm, mean weight of 80.12 ± 12.58 kg and mean body mass index (BMI) of 24.53 ± 3.60 kg/m2. All the team members, as the samples, were chosen via voluntary  sampling  method on a voluntary  basis. They were randomly  positioned  into two groups of training (n = 12) and cupping (n = 10). Serum concentrations  of creatine kinase (CK) and lactate dehydrogenase (LDH) were measured before and immediately and 24 hours after  the  training  and  cupping  using  the  enzyme-linked   immunosorbent   assay  (ELISA) methods.  Data  were  analyzed  using  independent-t  test  and  multi  variety.  There  was  a significant difference between the average concentration of the enzymes in before, next, and 24 hours after the test. According to the results of this study, the exercise and cupping caused muscle damage and increased the damage markers in blood https://jtim.tums.ac.ir/index.php/jtim/article/view/44 https://jtim.tums.ac.ir/index.php/jtim/article/download/44/58