Tehran University of Medical Sciences Traditional and Integrative Medicine 2476-5104 7 1 2022 03 09 1 12 EN Kamyar Khoshnevisan Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Hiva Alipanah Department of Physiology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran Hadi Baharifar Department of Medical Nanotechnology, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, 1477893855, Iran Negar Ranjbar Department of Medicine, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran Mahmoud Osanloo Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran 2021 05 30 2021 07 16 Cancer is the second leading cause of death worldwide, and due to the emergence of resistance to synthetic drugs in different cancers, developing new green drugs have become crucial. In this study, chitosan nanoparticles containing Cinnamomum verum J.Presl essential oil and cinnamaldehyde (major ingredient) were first prepared. The obtained nanoparticles were then characterized using Dynamic Light Scattering (DLS), Transmission electron microscopy (TEM), and Attenuated Total Reflection-Fourier Transform InfraRed (ATR-FTIR). After that, anticancer effects of the as-prepared nanoparticles were investigated. IC50 values of chitosan nanoparticles containing the essential oil were observed at 79 and 112 µg/mL against A-375 and MDA-MB-468 cells, respectively. These values for chitosan nanoparticles containing cinnamaldehyde were obtained at 135 and 166 µg/mL. The results of the current study indicated that chitosan nanoparticles containing C. verum essential oil can inhibit the growth of human melanoma (A-375) and breast cancer (MDA-MB-468) cells. https://jtim.tums.ac.ir/index.php/jtim/article/view/435