Tehran University of Medical Sciences Traditional and Integrative Medicine 2476-5104 1 3 2016 10 04 108 114 EN Arman Zargaran Department of Traditional Pharmacy, School of Persian Medicine, Tehran University of Medical Sciences, Tehran AND Pharmaceutical Sciences Research Center, Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Pouya Faridi Pharmaceutical Sciences Research Center, Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy AND Research Office for the History of Persian Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Saeid Daneshamouz Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Afshin Borhani-Haghighi Clinical Neurology Research Center AND Department of Neurology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Amir Azadi Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Mohammad-Hashem Hashempur Department of Traditional Medicine, Fasa University of Medical Sciences, Fasa, Iran Abdolali Mohagheghzadeh Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran 2016 10 02 2016 10 02 Medicinal oils were of the pharmaceutical dosage forms used since ancient Persian times to now in Iran. Chamomile  oil is one of the medicinal  oils prepared by the extraction of the chamomile flowers to sesame oil as an oily vehicle. It was widely used in the history of Persian medicine and is currently used by traditional practitioners of Persian Medicine in various disorders mainly in topical form. In this study, traditional chamomile oil was prepared based on the Qarabadin books. In advanced, 600 g of chamomile flower powder was boiled in 3.6 liter of water for 3 hours. Then, powder was removed and remained water (aqueous extract of chamomile) was boiled with 0.5 liter of sesame oil for 2 hours (until all the water was vaporized and oil remained). For standardization, the essential oil of chamomile oil was obtained via Clevenger apparatus method and then analyzed with the help of gas chromatography (GC)-mass method. In addition, total phenolic and flavonoid contents of the chamomile oil were calculated based on galic acid and quercetin, respectively. The results show that the main component of the essential oil were Caryophyllene (7.45%), Bisabolol Oxide  B (2.05%),  Bisabolone  Oxide  A (62.35%),  Chamazulene  (2.05%),  Bisabolol  Oxide  A (15.54%)  and Methyl ester 5,8,11-Heptadecatriynoic acid (5.52%). Besides, total phenolic and flavonoid contents were 11.0043 ± 0.4514 and 2.7640 ± 0.1776 mg/l, respectively.  Our results show that the historical dosage form of chamomile oil in Persian medicine can be reproduced and is an stable and homogeneous oil and be standardized in our investigation https://jtim.tums.ac.ir/index.php/jtim/article/view/43 https://jtim.tums.ac.ir/index.php/jtim/article/download/43/57