Tehran University of Medical Sciences Traditional and Integrative Medicine 2476-5104 7 1 2022 03 09 40 51 Abbas Joushan Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran Sadegh Rajabi Traditional Medicine and Materia Medica Research Center (TMRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran Khosrow Agin Pulmonologist and Pulmonary Critical Care, Shahid Beheshti University of Medical Sciences, Loghman Hakim General Teaching Hospital, Tehran, Iran Mohammad Hossein Ayati School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran Fatemeh Jafari Traditional Medicine and Materia Medica Research Center (TMRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran Babak Daneshfard Chronic Respiratory Diseases Research Center, Natoinal Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Persian Medicine Network (PMN), Universal Scientific Education and Research Network (USERN), Tehran, Iran Sayyed Shamsadin Athari Department of Immunology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Zeinab Ghahremani Department of Psychiatric Nursing, School of Nursing and Midwifery, Zanjan University of Medical Sciences, Zanjan, Iran Rasool Choopani Traditional Medicine and Materia Medica Research Center (TMRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2021 04 28 2021 07 14 As an inflammatory disease of the lung, asthma is characterized by bronchoconstriction, mucus hypersecretion, inflammatory mediator release, and eosinophil recruitment. Cupping therapy is an ancient method of treatment for a vast range of ailments. This study aimed to evaluate the anti-asthmatic effects of wet cupping therapy (WCT) in a mouse model. A total number of 35 Balb/c mice were randomly divided into five groups (n = 7): Negative and positive control groups were administered Phosphate Buffered Saline (PBS) and ovalbumin (OVA), respectively. The remaining three OVA-challenged groups were treated with budesonide, one session, and two sessions of WCT. Finally, eosinophil counts, the gene expressions, and the protein levels of interleukins IL-5, -13, and -33 were measured in bronchoalveolar lavage fluid (BALF) of mice. Lung tissues were removed and kept for histopathological evaluations. Both eosinophil counts and interleukin levels in BALFs were significantly diminished following WCT. Moreover, WCT prevented hyperplastic growth of goblet cells, overproduction of mucus, and inflammation of peribronchial and perivascular areas of lung tissue of mice compared to positive control group. Interestingly, the anti-inflammatory effects of WCT against asthma were comparable to budesonide. Our data suggested that the anti-asthmatic effects of WCT were mediated by reducing eosinophil trafficking and modulating Th2 inflammatory cytokines, leading to the histological changes of the lung. This may propose WCT as an efficient therapeutic approach to mitigate inflammatory complications of asthma. https://jtim.tums.ac.ir/index.php/jtim/article/view/404