Alpha-amylase Inhibitory Activity of Sitopaladi Churna: An Ayurvedic Formula

  • Kolavanahalli Pillegowda-Smitha Department of Pharmaceutics, Sri K.V. College of Pharmacy, Chickballapur, Karnataka, India
  • Kuruba Srinivas-Kulashekar Department of Pharmacology, Sri K.V. College of Pharmacy, Chickballapur, Karnataka, India
  • Thotlaganahalli Krishnappa-Pavithra Department of Pharmaceutics, Sri K.V. College of Pharmacy, Chickballapur, Karnataka, India
  • Bagepalli Srinivas-Ashok-Kumar Department of Pharmacognosy, Sri K.V. College of Pharmacy, Chickballapur, Karnataka, India
  • Gopi Setty-Saran Department of Pharmaceutics, Sri K.V. College of Pharmacy, Chickballapur, Karnataka, India
Keywords: Sitopaladi Churna, Ayurvedic Formula, Diabetes, Alpha Amylase, Dinitrosalicylic Acid

Abstract

Sitopaladi Churna is an ayurvedic formula, traditionally used as anti-inflammatory, carminative, sedative, expectorant, anti-infective, and various respiratory disorders. The aim of the present study is to screening for alpha amylase (α-amylase) inhibition activity of aqueous extract of Sitopaladi Churna. In vitro α-amylase inhibition activity of Sitopaladi Churna was screened by the 3,5-dinitrosalicylic acid method. Sitopaladi Churna showed potent (α-amylase) inhibitory activity with an IC50  - 46.38 µ g/ml. Thus, Sitopaladi Churna may consider as a remedy for diabetes and other  insulin  resistance-related  diseases;  however,  animal  and  human  studies  are  needed  to confirm this activity.

References

Marshall JJ, Lauda CM. Purification and properties of phaseolamin, an inhibitor of alpha-amylase, from the kidney bean, Phaseolus vulgaris. J Biol Chem 1975; 250(20): 8030-7.

Puls W, Keup U. Influence of an -amylase inhibitor (BAY d 7791) on blood glucose, serum insulin and NEFA in starch loading tests in rats, dogs and man. Diabetologia 1973; 9(2): 97-101.

Layer P, Zinsmeister AR, DiMagno EP. Effects of decreasing intraluminal amylase activity on starch digestion and postprandial gastrointestinal function in humans. Gastroenterology 1986; 91(1): 41-8.

Ramalingayya M, Sastry MV. Vaidya yoga ratnavali = Formulary of Ayurvedic medicines. Chennai, India: Indian Medical Practitioners' Co- operative Pharmacy & Stores; 2000. p. 123.

Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 1959; 31(3): 426-8.

Punitha R, Manoharan S. Antihyperglycemic and antilipidperoxidative effects of Pongamia pinnata (Linn.) Pierre flowers in alloxan induced diabetic rats. J Ethnopharmacol 2006; 105(1-2): 39-46.

Kwon YI, Vattem DA, Shetty K. Evaluation of clonal herbs of Lamiaceae species for management of diabetes and hypertension. Asia Pac J Clin Nutr 2006; 15(1): 107-18.

Folsch UR, Creutzfeldt W. Adaptation of the pancreas during treatment with enzyme inhibitors in rats and man. Scand J Gastroenterol Suppl 1985; 112: 54-63.

Chopra RN. Indigenous drugs of India. 2nd ed. Kolkata, India: U. N. Dhur & Sons Pvt. LTD; 1958. p. 289, 665.

Khare CP. Indian medicinal plants: an illustrated dictionary. New York, NY: Springer Science & Business Media; 2008. p. 90.

Muniappan M, Sundararaj T. Antiinflammatory and antiulcer activities of Bambusa arundinacea. J Ethnopharmacol 2003; 88(2-3): 161-7.

Pérez Trueba LG. Los flavonoides: antioxidantes o prooxidantes. Rev Cubana Invest Biomed 2003; 42 Trad Integr Med 2015; Vol. 1, No. 1 Kumar, et al. 22(1): 48-57.

Vanithakumari G, Manonayagi S, Padma S, Malini T. Antifertility effect of Bambusa arundinacea shoot extracts in male rats. J Ethnopharmacol 1989; 25(2): 173-80.

Jayaprakasha GK, Jagan Mohan RL, Sakariah KK. Volatile constituents from Cinnamomum zeylanicum fruit stalks and their antioxidant activities. J Agric Food Chem 2003; 51(15): 4344-8.

Kar A, Choudhary BK, Bandyopadhyay NG. Comparative evaluation of hypoglycaemic activity of some Indian medicinal plants in Alloxan diabetic rats. J Ethnopharmacol 2003; 84(1): 105-8.

Verspohl EJ, Bauer K, Neddermann E. Antidiabetic effect of Cinnamomum cassia and Cinnamomum zeylanicum in vivo and in vitro. Phytother Res 2005; 19(3): 203-6.

Sengupta A, Ghosh S, Bhattacharjee S. Dietary cardamom inhibits the formation of azoxymethane- induced aberrant crypt foci in mice and reduces COX-2 and iNOS expression in the colon. Asian Pac J Cancer Prev 2005; 6(2): 118-22.

Al-Harthi MA. Impact of supplemental feed enzymes, condiments mixture or their combination on broiler performance, nutrients digestibility and plasma constituents. International Journal of Poultry Science 2006; 5(8): 764-71.

Singh G, Kiran S, Marimuthu P, Isidorov V, Vinogorova V. Antioxidant and antimicrobial activities of essential oil and various oleoresins of Elettaria cardamomum (seeds and pods). Journal of the Science of Food and Agriculture 2008; 88(2): 280-9.

Verma SK, Jain V, Katewa SS. Blood pressure lowering, fibrinolysis enhancing and antioxidant activities of cardamom (Elettaria cardamomum). Indian J Biochem Biophys 2009; 46(6): 503-6.

Dev S. A selection of prime ayurvedic plant drugs: ancient-modern concordance. Delhi, Indian: Anamaya Publishers; 2006.

Manoharan S, Silvan S, Vasudevan K, Balakrishnan S. Antihyperglycemic and antilipidperoxidative effects of piper longum (Linn.) dried fruits in Alloxan induced diabetic rat. Journal of Biological Sciences 2007; 7: 161-8.

Published
2015-11-11
How to Cite
1.
Pillegowda-Smitha K, Srinivas-Kulashekar K, Krishnappa-Pavithra T, Srinivas-Ashok-Kumar B, Setty-Saran G. Alpha-amylase Inhibitory Activity of Sitopaladi Churna: An Ayurvedic Formula. Trad Integr Med. 1(1):40-43.
Section
Research Article(s)