Campanula latifola, Giant Bellflower; Ethno-botany, Phytochemical and Antioxidant Evaluation

  • Seyed Reza Moosavi Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  • Mohammad Reza Shams Ardekani Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  • Mahdi Vazirian Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  • Seyede Nargess Sadati Lamardi Department of Traditional Pharmacy, School of Iranian Medicine, Tehran University of Medical Sciences, Tehran, Iran
Keywords: Campanula latifolia; Phytochemical evaluation; Antioxidant; DPPH; Folin Ciocalteu

Abstract

Campanula latifolia is a plant of Campanulaceae family, commonly known as Giant bellflower. Campanula genus is mentioned in Iranian medicine sources with various names such as kaff- e- Maryam and Gol-e-estekani. The aim of this study was to assay the different groups of chemical compounds and to investigate the antioxidant effects of ethanolic extract of aerial parts and root of this plant. After providing 70% alcoholic extract of aerial parts and root, qualitative tests were performed to evaluate anthocyanins, saponins, cardiac glycosides, flavonoids, tannins, sterols, triterpenoids, and alkaloids. Tests of antioxidant effects were evaluated by DPPH free radical scavenging effect and the content of phenolic compounds in the extracts using Folin-Ciocalteu method. Phytochemical studies indicate that there are compounds of cardiac glycoside, triterpene, and sterols. In the quantitative analysis of the compounds of the two plant extracts, the total amount of phenols were measured 0.053 and 0.037 (mg Gallic acid/g extract) in aerial parts and root extracts, respectively. The amount of IC50 of DPPH radical scavenging activity of the plant has also been 1163.636 μg / ml for the aerial parts extract and 1419.354 μg / ml for the root extract. Considering the results and the presence of cardiac glycosides, triterpenoids, and sterols, further phytochemical and pharmacological studies are suggested.

References

[1] Flora of China. Campanula Linnaeus, Sp. Pl. 1: 163. 1753. 2011; 19: 530. http://www.efloras.org/florataxon.aspx?flora_id=2&taxon_id=105395
[2] J Kookhdan A. Taxonomic evaluation of Campanulaceae family in Iran. [M.S. dissertation][In Persian]. Tehran University of Sciences, Tehran; 1985.
[3] "Giant Bellflower: Campanula latifolia". NatureGate. Retrieved 2013-12-31. http://www.luontoportti.com/suomi/en/kukkakasvit/giant-bellflower
[4] Advay M, Maroofi H. A new species of campanula from kurdistan, west of Iran. Iran J Bot 2015;21:35-38.
[5] Mozafan V. Identification of medicinal plants and aromatic plants of Iran. Farhang Moaser. Tehran 2013; p 110.
[6] Soltani A. Encyclopedia of traditional medicine (Dictionary of medicinal plants).Vol. 2 (C-D). Arjomand Press. Tehran 2005; pp 54-56.
[7] Medicinal Plants. Campanula (Bellflower) Species. 2015. From http://medicinalplants.us/campanula-bellflower-species
[8] Usta, CananYildirim, Arzu Birinci Turker, Arzu Ucar. Antibacterial and antitumour activities of some plants grown in Turkey. Biotechnol Biotechnol Equip 2014;28:306-315.
[9] Politeo O, Skocibusic M, Burcul F, Maravic A, Carev I, Ruscic M, Milos M. Campanula portenschlagiana ROEM. et SCHULT. Chemical and antimicrobial activities. Chem Biodivers 2013;10:1072-1080.
[10] Park SH, Sim YB, Lim SS, Kim JK, Lee JK, Suh HW. Antinociception effect and mechanisms of Campanula punctata extract in the mouse. Korean J Physiol Pharmacol 2010 ;14:285-289.
[11] Wagner H, Bladt S. Plant Drug Analysis. 2ed ed. Spring¬er-Verlag. Berlin 1996; p 384.
[12] Velioglu Y, Mazza G, Gao L, Oomah B. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 1998;46:4113-4117.
[13] Vazirian M, Vosughian E, Pirali-Hamedani M, Khodaei M, Sadati Lamardi SN. Essential oil composition and radical scavenging activity of Paeonia daurica Subsp. macrophylla root . Trad Integr Med 2018;3:11-17.
[14] Guarrera PM. Food medicine and minor nourishment in the folk traditions of Central Italy (Marche, Abruzzo and Latium). Fitoterapia 2003;74:515-544.
[15] Ranfa A, Bodesmo M. An Ethnobotanical investigation of traditional knowledge and uses of edible wild plants in the Umbria Region, Central Italy. JABFQ 2017;90:246-258.
[16] Al-Quran S. Taxonomical and pharmacological survey of therapeutic plants in Jordan. J Nat Prod 2008;1:10-26.
[17] Ghirardini MP, Carli M, Del Vecchio N, Rovati A, Cova O, Valigi F. The importance of a taste. A comparative study on wild food plant consumption in twenty-one local communities in Italy. J Ethnobiol Ethnomed 2007;3:22.
[18] Mаrković M, Mаtović M, Pаvlović D, Zlаtković B, Mаrković A, Jotić B. Resources of medicinal plants and herbs collector’s calendar of Pirot County (Serbia). Biologica Nyssana 2010;1:9-21.
[19] Parihaar RS, Bargali K, Bargali SS. Diversity and uses of Ethno-medicinal plants associated with traditional agroforestry systems in Kumaun Himalaya. Indian Journal of Agricultural Sciences 2014;84:1470-1476.
[20] Dogan Y, Baslar S, Ay G, Mert HH. The use of wild edible plants in western and central Anatolia (Turkey). Economic Botany 2004;58:684-690.
[21] Usta C, Yildirim AB, Turker AU. Antibacterial and antitumour activities of some plants grown in Turkey. Biotechnology & Biotechnological Equipment 2014;28:306-315.
[22] Dzhumyrko S F. Coumarins of species of the genus Campanula. Chem Nat Compd 1984; 20:616-617.
[23] Dzhumyrko, S. F. Flavonoids of species of the genus Campanula. Chem Nat Compd 1985; 21:531-532.
[24] Yayli N, Yildirim N, Doğan N, Usta A, Altun L. Triterpenes from Campanula lactiflora. J Asian Nat Prod Res 2005;7:771-775.
[25] Cuendet M, Potterat O, Hostettmann K. Flavonoids and phenylpropanoid derivatives from Campanula barbata. Phytochemistry 2001;56:631-636.
[26] Dumlu MU, Gurkan E, Tuzlaci E. Chemical composition and antioxidant activity of Campanula alliariifolia. Nat Prod Res 2008;22:477-482.
[27] Jaradat NA, Abualhasan M. Comparison in vitro of antioxidant activity between aifteen Campanula species (Bellflower) from Palestinian flora. Pharmacogn J 2015;7:276-279.
Published
2018-10-01
How to Cite
1.
Moosavi SR, Shams Ardekani MR, Vazirian M, Sadati Lamardi SN. Campanula latifola, Giant Bellflower; Ethno-botany, Phytochemical and Antioxidant Evaluation. JTIM. 3(3):113-9.
Section
Research Article(s)